SingleFlowEx CD22 Alexa Fluor® 700

SingleFlowEx CD22 Alexa Fluor<sup>®</sup> 700
Regulatory status
RUO (GMP)
Antigen
CD22
Clone
S-HCL-1
Format
Alexa Fluor® 700
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
1 ml
ED7712
In stock
440.00 USD
Variant
0.1 mg
11-261-C100
In stock
440.00 USD
Product details
Images
References
Isotype
Mouse IgG2b kappa
Specificity
CD22
Application
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.025 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
ED7712_FC_Dot-plot
Example of surface staining pattern using SingleFlowEx CD22 Alexa Fluor® 700
Clone: S-HCL-1
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx CD22 Alexa Fluor® 700 versus CD19 PE
ED7712_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD22 Alexa Fluor® 700
Clone: S-HCL-1
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: CD22 positive CD19 positive lymphocytes
Negative events: Neutrophil granulocytes
ED7712_FC_Profil Značení
Example of surface staining pattern using SingleFlowEx CD22 Alexa Fluor® 700
Clone: S-HCL-1
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)

Product specific references:

Use of this product has not been published.
Variant
1 ml
ED7712
In stock
440.00 USD
Variant
0.1 mg
11-261-C100
In stock
440.00 USD