SingleFlowEx CD71 APC-Cy™7

SingleFlowEx CD71 APC-Cy™7
Regulatory status
RUO (GMP)
Antigen
CD71
Clone
MEM-75
Format
APC-Cy™7
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
1 ml
ED7165
Delivery 1 week
550.00 USD
Variant
1 ml
ED7165
Delivery 1 week
550.00 USD
Product details
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
CD71
Application
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.050 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
ED7165_FC_Dot-plot
Example of surface staining pattern using SingleFlowEx CD71 APC-Cy™7
Clone: MEM-75
Sample: EDTA-anticoagulated human peripheral blood from healthy donor 100x diluted in 1× PBS + 0.5% BSA
Staining protocol: 10 μl reagent / 100 μl of diluted blood sample; extracellular erythrocyte staining wash protocol
Graphic display: all events displayed as SingleFlowEx CD71 APC-Cy™7 versus CD235a FITC
ED7165_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD71 APC-Cy™7
Clone: MEM-75
Sample: EDTA-anticoagulated human peripheral blood from healthy donor 100x diluted in 1× PBS + 0.5% BSA
Staining protocol: 10 μl reagent / 100 μl of diluted blood sample; extracellular erythrocyte staining wash protocol
Positive events: CD71 positive CD235a positive reticulocytes
Negative events: CD71 negative CD235a positive erythrocytes
ED7165_FC_Profil Značení
Example of surface staining pattern using SingleFlowEx CD71 APC-Cy™7
Clone: MEM-75
Sample: EDTA-anticoagulated human peripheral blood from healthy donor 100x diluted in 1× PBS + 0.5% BSA
Staining protocol: 10 μl reagent / 100 μl of diluted blood sample; extracellular erythrocyte staining wash protocol

Product specific references:

Use of this product has not been published.
Variant
1 ml
ED7165
Delivery 1 week
550.00 USD
Variant
1 ml
ED7165
Delivery 1 week
550.00 USD