Exbio — Clinical products — Immunodeficiency — Single color antibodies — SingleFlowEx CD71 APC-Cy™7

SingleFlowEx CD71 APC-Cy™7

Regulatory status

RUO (GMP)

Antigen

CD71

Clone

MEM-75

Format

APC-Cy™7

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

1 ml

ED7165

Delivery 1 week

550.00 USD

Variant

1 ml

ED7165

Delivery 1 week

550.00 USD

Contact distributor

Product details

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

CD71

Application

FC (QC tested)

Reactivity

Human

Description

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Contents

1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.

Concentration

0.050 mg/ml

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Example of surface staining pattern using SingleFlowEx CD71 APC-Cy™7
Clone: MEM-75
Sample: EDTA-anticoagulated human peripheral blood from healthy donor 100x diluted in 1× PBS + 0.5% BSA
Staining protocol: 10 μl reagent / 100 μl of diluted blood sample; extracellular erythrocyte staining wash protocol
Graphic display: all events displayed as SingleFlowEx CD71 APC-Cy™7 versus CD235a FITC

Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD71 APC-Cy™7
Clone: MEM-75
Sample: EDTA-anticoagulated human peripheral blood from healthy donor 100x diluted in 1× PBS + 0.5% BSA
Staining protocol: 10 μl reagent / 100 μl of diluted blood sample; extracellular erythrocyte staining wash protocol
Positive events: CD71 positive CD235a positive reticulocytes
Negative events: CD71 negative CD235a positive erythrocytes