SingleFlowEx CD71 APC

SingleFlowEx CD71 APC
Regulatory status
RUO (GMP)
Antigen
CD71
Clone
MEM-75
Format
APC
Reactivity
Human
Application
FC
Excitation laser
red (633 nm)
Variant
1 ml
ED7660
Out of stock
440.00 USD
Variant
0.1 mg
11-261-C100
In stock
440.00 USD
Product details
Images
References
Isotype
Mouse IgG1
Specificity
CD71
Application
FC
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.050 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
The product is shipped with ice packs. Transport conditions, including short-term elevated temperature, do not impair product performance characteristics or do not affect stated product expiry date. Upon receipt, store and handle as directed.
Store at 2-8°C.
Keep away from sunlight.
Do not freeze.
Do not use after the expiry date stated on the product labels.
No reconstitution is necessary. Use the product directly from its original product container.
The product retains its performance characteristics until the expiry date when stored under stated conditions in its original product container.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
ED7660_FC_Dot-plot
Example of surface staining pattern using SingleFlowEx CD71 APC
Clone: MEM-75
Sample: EDTA-anticoagulated human peripheral blood from healthy donor 100x diluted in 1× PBS + 0.5% BSA
Staining protocol: 10 μl reagent / 100 μl of diluted blood sample; extracellular erythrocyte staining wash protocol
Graphic display: all events displayed as SingleFlowEx CD71 APC versus CD235a FITC
ED7660_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD71 APC
Clone: MEM-75
Sample: EDTA-anticoagulated human peripheral blood from healthy donor 100x diluted in 1× PBS + 0.5% BSA
Staining protocol: 10 μl reagent / 100 μl of diluted blood sample; extracellular erythrocyte staining wash protocol
Positive events: CD71 positive CD235a positive reticulocytes
Negative events: CD71 negative CD235a positive erythrocytes
ED7660_FC_Profil Značení
Example of surface staining pattern using SingleFlowEx CD71 APC
Clone: MEM-75
Sample: EDTA-anticoagulated human peripheral blood from healthy donor 100x diluted in 1× PBS + 0.5% BSA
Staining protocol: 10 μl reagent / 100 μl of diluted blood sample; extracellular erythrocyte staining wash protocol

Product specific references:

Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2020 Mar;98(2):179-192
PubMed
Variant
1 ml
ED7660
Out of stock
440.00 USD
Variant
0.1 mg
11-261-C100
In stock
440.00 USD