SingleFlowEx CD34 PerCP-Cy™5.5

SingleFlowEx CD34 PerCP-Cy™5.5
Regulatory status
RUO (GMP)
Antigen
CD34
Clone
4H11[APG]
Format
PerCP-Cy™5.5
Reactivity
Human
Application
FC
Excitation laser
blue (488 nm)
Variant
1 ml
ED7666
Out of stock
600.00 USD
Variant
0.1 mg
11-261-C100
In stock
600.00 USD
Product details
Images
Isotype
Mouse IgG1
Specificity
CD34
Application
FC
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.030 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
The product is shipped with ice packs. Transport conditions, including short-term elevated temperature, do not impair product performance characteristics or do not affect stated product expiry date. Upon receipt, store and handle as directed.
Store at 2-8°C.
Keep away from sunlight.
Do not freeze.
Do not use after the expiry date stated on the product labels.
No reconstitution is necessary. Use the product directly from its original product container.
The product retains its performance characteristics until the expiry date when stored under stated conditions in its original product container.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of GE Healthcare.
ED7666_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD34 PerCP-Cy™5.5
Clone: 4H11[APG]
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: CD34 positive CD45 dim hematopoietic stem cells
Negative events: Lymphocytes
ED7666_FC_Profil Značení
Example of surface staining pattern using SingleFlowEx CD34 PerCP-Cy™5.5
Clone: 4H11[APG]
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
ED7666_FC_Profil Značení_2
Example of surface staining pattern using SingleFlowEx CD34 PerCP-Cy™5.5
Clone: 4H11[APG]
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Blue dots: All events
Red dots: CD34 positive CD45 dim hematopoietic stem cells
Variant
1 ml
ED7666
Out of stock
600.00 USD
Variant
0.1 mg
11-261-C100
In stock
600.00 USD