SingleFlowEx CD34 Pacific Blue™

SingleFlowEx CD34 Pacific Blue™
Regulatory status
RUO (GMP)
Antigen
CD34
Clone
581
Format
Pacific Blue™
Reactivity
Human
Application
Excitation laser
violet (405 nm)
Variant
1 ml
ED7543
Delivery 1 week
484.00 USD
Variant
1 ml
ED7543
Delivery 1 week
484.00 USD
Product details
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
CD34
Application
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.030 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
ED7543_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD34 Pacific Blue™
Clone: 581
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: CD34 positive CD45 dim hematopoietic stem cells
Negative events: Lymphocytes
ED7543_FC_Profil Značení
Example of surface staining pattern using SingleFlowEx CD34 Pacific Blue™
Clone: 581
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
ED7543_FC_Profil Značení_2
Example of surface staining pattern using SingleFlowEx CD34 Pacific Blue™
Clone: 581
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Blue dots: All events
Red dots: CD34 positive CD45 dim hematopoietic stem cells

Product specific references:

Use of this product has not been published.
Variant
1 ml
ED7543
Delivery 1 week
484.00 USD
Variant
1 ml
ED7543
Delivery 1 week
484.00 USD