CD20 FITC

CD20 FITC
Regulatory status
CE IVD
Antigen
CD20
Format
FITC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
ED7005
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Product details
References
Application
FC (QC tested)
Reactivity
Human
Description
The reagent CD20 FITC permits identification and enumeration of cell populations expressing human CD20 antigen in whole blood using flow cytometry.
Contents
The reagent contains mouse monoclonal antibody against human CD20 antigen (clone LT20) which was purified by affinity chromatography and labeled with Fluorescein isothiocyanate (FITC). The labeled antibody is diluted in an optimal concentration in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. The content of a vial (2 ml) is sufficient for 100 tests.
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended for In Vitro Diagnostic Use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Product specific references:

Glennie MJ et al. (2007) Mechanisms of killing by anti-CD20 monoclonal antibodies. Mol Immunol. 44: 3823-37
Teeling JL et al. (2006) The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20. J Immunol. 177: 362-71
Cragg MS et al. (2005) The biology of CD20 and its potential as a target for mAb therapy. Curr Dir Autoimmun. 8: 140-174
Janas E et al. (2005) Functional role of lipid rafts in CD20 activity? Biochem Soc Symp. 72: 165-75
Li H et al. (2004) The CD20 calcium channel is localized to microvilli and constitutively associated with membrane rafts. J Biol Chem. 279: 19893-19901
Chan HT et al. (2003) CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts. Cancer Res. 63: 5480-9
Petrie RJ and Deans JP (2002) Colocalization of the B cell receptor and CD20 followed by activation-dependent dissociation in distinct lipid rafts. J. Immunol. 169: 2886-2891
Polyak MJ et al. (2002) Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood. 99: 3256-62
Variant
100 tests
ED7005
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD