Exbio — Clinical products — Immunodeficiency — Lysing solutions — Monocyte Blocking Buffer

Monocyte Blocking Buffer

Regulatory status

RUO

Application

FC (QC tested)

Variant

1 ml

ED7747

In stock

138.00 USD

Variant

1 ml

ED7747

In stock

138.00 USD

Contact distributor

Product details

Images

SDS download

Application

FC (QC tested)

Description

The reagent minimizes monocyte background in immunophenotyping procedures that involve the use of tandem dye monoclonal antibody conjugates.

Contents

The content of the vial (1 ml) is sufficient for 100 staining reactions. The solution is ready to use.

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Flow cytometry multicolor surface staining patterns of human leukocytes stained using anti-human CD19 (LT19) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD14 (MEM-15) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).
The picture on the left shows staining pattern without the use of Monocyte Blocking Buffer. The picture on the right shows staining pattern with the use of Monocyte Blocking Buffer (10 µl reagent / 100 μl of peripheral whole blood).
Only non-specific binding of tandem fluorochromes to monocytes is affected, specific binding of monocyte-marker CD14 is not affected by the use of Monocyte Blocking Buffer.

Flow cytometry surface staining patterns of human peripheral whole blood stained using anti-human CD19 (LT19) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).
The picture on the left shows staining pattern without the use of Monocyte Blocking Buffer. The picture on the right shows staining pattern with the use of Monocyte Blocking Buffer (10 µl reagent / 100 μl of peripheral whole blood).