Exbio — Clinical products — Immunodeficiency — Kits — T-cell BlastoFlowEx Kit

T-cell BlastoFlowEx Kit

Regulatory status

RUO

Reactivity

Human

Application

FC (QC tested)

Variant

100 tests

ED7642

In stock

393.00 USD

Variant

100 tests

ED7642

In stock

393.00 USD

Contact distributor

Product details

Images

IFU download

SDS download

Application

FC (QC tested)

Reactivity

Human

Description

The T-cell BlastoFlowEx Kit is designed to measure the proliferative response of T-lymphocytes in activated samples of whole human blood. The kit uses anti-CD3 and anti-Ki67 antibody cocktail to detect the proliferating lymphocytes.

Contents

ED7642-1 Fix and Lysing Solution, 1x 25ml, intended to prepare 250 ml of 1x solution (2 ml per test).
ED7642-2 Permeabilizing Solution, 1x 10 ml, intended to prepare 100 ml of 1x solution (0.5 ml per test).
ED7642-3 EDTA, 1x 2.5ml (0.025 ml per test).
ED7642-4 CD3 APC/Ki-67 PE, 2x 2.5ml (0.05ml per test).

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Whole blood from Cytomegalovirus positive donor was stimulated in vitro with antigenic peptide derived from Cytomegalovirus - IE-1 protein. The proliferative response of T-lymphocytes was detected with T-cell BlastoFlowEx Kit.

Whole blood from Cytomegalovirus positive donor was stimulated in vitro with antigenic peptide derived from Cytomegalovirus - pp65 protein. The proliferative response of T-lymphocytes was detected with T-cell BlastoFlowEx Kit.

Whole blood was stimulated in Phytohemagglutinin (PHA) tube (Cat. No. ED7634), processed with T-cell BlastoFlowEx Kit and analyzed in a flow cytometer. All ungated events were plotted with side scatter on the Y-axis vs forward scatter on the X axis with the threshold set on forward scatter (vertical dotted line). The target subpopulation of CD3+/Single Cells was displayed as a histogram of PE fluorescence. The discrimination line between the Ki-67 negative and Ki-67 positive peak was determined according to the Stimulation Negative Control tube (Cat. No. ED7637).

Further IFU language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
ED7642_TDS_v7_CS.pdf
ED7642_TDS_v7_EN.pdf