Exbio — Clinical products — Immunodeficiency — Kits — FagoFlowEx Kit

FagoFlowEx Kit

Regulatory status

CE IVD

Reactivity

Human

Application

FC (QC tested)

Variant

100 tests

ED7042

In stock

462.00 USD

Variant

100 tests

ED7042

In stock

462.00 USD

Contact distributor

Product details

Images

References

IFU download

SDS download

Application

FC (QC tested)

Reactivity

Human

Description

The FagoFlowEx Kit is intended for the examination of phagocytic activity of neutrophil granulocytes by measuring the respiratory (oxidative) burst after their stimulation with E. coli bacteria in human heparinized whole blood using flow cytometry.

Contents

ED7042-1 E.coli – 5 vials containing lyophilized E. coli bacteria, 1 vial is intended for stimulation of 20 tubes.
ED7042-2 DHR123 – 5 vials containing lyophilized Dihydrorhodamine 123, 1 vial is intended for staining of 60 tubes.
ED7042-3 Stimulation Control – 5 vials containing lyophilized PMA (Phorbol 12-myristate 13-acetate), 1 vial is intended for stimulation of 20 positive controls.
ED7042-4 Lysing Solution – 15 ml

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended for In Vitro Diagnostic Use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Separation of stimulated neutrophil granulocytes (red-filled) from non-stimulated neutrophil granulocytes (negative control; black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood of donor with chronic granulomatous disease (CGD), causing defect of respiratory burst (SI = 11), stained using FagoFlowEx Kit.

Separation of stimulated neutrophil granulocytes (red-filled) from non-stimulated neutrophil granulocytes (negative control; black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood of healthy donor without defect of respiratory burst (SI = 98), stained using FagoFlowEx Kit.

Histograms showing respirátory burst in negative control (left), positive control (middle) and E. coli stimulated sample (right) of human peripheral whole blood of healthy donor without defect of respiratory burst, stained using FagoFlowEx Kit.

Separation of stimulated neutrophil granulocytes (red-filled) from non-stimulated neutrophil granulocytes (negative control; black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood of female donor with heterozygotic mutation of X-linked gene causing chronic granulomatous disease (CGD), which causes defect of respiratory burst in half of neutrophil granulocytes, stained using FagoFlowEx Kit. Two granulocyte subpopulations differ in respirátory burst intensity, SI (low) = 13.9, SI (high) = 125.

Separation of stimulated neutrophil granulocytes (red-filled) from non-stimulated neutrophil granulocytes (negative control; black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood of donor with myeloperoxidase deficiency, causing defect of respiratory burst, stained using FagoFlowEx Kit. The stimulation index is reduced approximately to 1/10 of normal value (SI = 11).

General references:

Heyworth P, Cross A, Curnutte J (2003) Chronic granulomatous disease. Curr Opin Immunol 15(5): 578-84
PubMed

Winkelstein J, Marino M, Johnston R et al (2000) Chronic granulomatous disease. Report on a national registry of 368 patients. Medicine (Baltimore) 79 (3):155-69
PubMed

Sawyer DW, Donowitz GR, Mandell GL (1989) Polymorphonuclear neutrophils: An effective antimicrobial force. Rev Infect Dis 11: 1532-44
PubMed

Product specific references:

Jbeily N, Suckert I, Gonnert FA, Acht B, Bockmeyer CL, Grossmann SD, Blaess MF, Lueth A, Deigner HP, Bauer M, Claus RA: Hyperresponsiveness of mice deficient in plasma-secreted sphingomyelinase reveals its pivotal role in early phase of host response. J Lipid Res. 2013 Feb;54(2):410-24.
PubMed

Jeraiby M, Sidi Yahya K, Depince-Berger AE, Lambert C: Microbicidal activity measured by flow cytometry: Optimization and standardization for detection of primary and functional deficiencies. J Immunol Methods. 2016 Sep 29. pii: S0022-1759(16)30220-4.
PubMed

Porpodis K, Domvri K, Zarogoulidis P, Petridis D, Tsirgogianni K, Papaioannou A, Hatzizisi O, Kioumis I, Liaka A, Kikidaki V, Lampaki S, Organtzis J, Zarogoulidis K: Roflumilast, a phosphodiesterase-4 inhibitor, induces phagocytic activity in Greek COPD patients. Int J Chron Obstruct Pulmon Dis. 2015 Jun 15;10:1123-8.
PubMed

Hromádková L, Zárybnická L, Vávrová J, Bílková Z, Šinkorová Z: The impact of pre-analytical treatment and sorting on human neutrophil function. Veterinární Medicína, 59, 2014 (11): 543-552
PubMed