FagoFlowEx Kit

FagoFlowEx Kit
Regulatory status
CE IVD
Reactivity
Human
Application
FC (QC tested)
Variant
100 tests
ED7042
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
Images
References
Application
FC (QC tested)
Reactivity
Human
Description
The FagoFlowEx Kit is intended for the examination of phagocytic activity of neutrophil granulocytes by measuring the respiratory (oxidative) burst after their stimulation with E. coli bacteria in human heparinized whole blood using flow cytometry.
Contents
ED7042-1 E.coli – 5 vials containing lyophilized E. coli bacteria, 1 vial is intended for stimulation of 20 tubes.
ED7042-2 DHR123 – 5 vials containing lyophilized Dihydrorhodamine 123, 1 vial is intended for staining of 60 tubes.
ED7042-3 Stimulation Control – 5 vials containing lyophilized PMA (Phorbol 12-myristate 13-acetate), 1 vial is intended for stimulation of 20 positive controls.
ED7042-4 Lysing Solution – 15 ml
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
ED7042_Histogram_Healthy
Separation of stimulated Neutrophil granulocytes (red-filled) from non-stimulated Neutrophil granulocytes (negative control; black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood of healthy donor without defect of respiratory burst, stained using FagoFlowEx® Kit.

General references:

Heyworth P, Cross A, Curnutte J (2003) Chronic granulomatous disease. Curr Opin Immunol 15(5): 578-84
PubMed
Winkelstein J, Marino M, Johnston R et al (2000) Chronic granulomatous disease. Report on a national registry of 368 patients. Medicine (Baltimore) 79 (3):155-69
PubMed
Sawyer DW, Donowitz GR, Mandell GL (1989) Polymorphonuclear neutrophils: An effective antimicrobial force. Rev Infect Dis 11: 1532-44
PubMed

Product specific references:

Jbeily N, Suckert I, Gonnert FA, Acht B, Bockmeyer CL, Grossmann SD, Blaess MF, Lueth A, Deigner HP, Bauer M, Claus RA: Hyperresponsiveness of mice deficient in plasma-secreted sphingomyelinase reveals its pivotal role in early phase of host response. J Lipid Res. 2013 Feb;54(2):410-24.
PubMed
Jeraiby M, Sidi Yahya K, Depince-Berger AE, Lambert C: Microbicidal activity measured by flow cytometry: Optimization and standardization for detection of primary and functional deficiencies. J Immunol Methods. 2016 Sep 29. pii: S0022-1759(16)30220-4.
PubMed
Porpodis K, Domvri K, Zarogoulidis P, Petridis D, Tsirgogianni K, Papaioannou A, Hatzizisi O, Kioumis I, Liaka A, Kikidaki V, Lampaki S, Organtzis J, Zarogoulidis K: Roflumilast, a phosphodiesterase-4 inhibitor, induces phagocytic activity in Greek COPD patients. Int J Chron Obstruct Pulmon Dis. 2015 Jun 15;10:1123-8.
PubMed
Hromádková L, Zárybnická L, Vávrová J, Bílková Z, Šinkorová Z: The impact of pre-analytical treatment and sorting on human neutrophil function. Veterinární Medicína, 59, 2014 (11): 543-552
PubMed
Variant
100 tests
ED7042
In stock
Variant
0.1 mg
11-261-C100
In stock