Proaerolysin Alexa Fluor® 488

Proaerolysin Alexa Fluor<sup>®</sup> 488
Regulatory status
RUO (GMP)
Antigen
Proaerolysin
Format
Alexa Fluor® 488
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
1 ml
ED7751
In stock
396.00 USD
Variant
1 ml
ED7751
In stock
396.00 USD
Product details
Images
References
SDS download
Specificity
Proaerolysin
Application
Reactivity
Human
Description
Proaerolysin Alexa Fluor® 488 is a fluorochrome-labeled recombinant protein single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine GPI deficiency in human clinical samples.
Proaerolysin Alexa Fluor® 488 can be used in various multicolor combinations. Laboratory shall establish its performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 200 tests
Concentration
0.075 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
ED7751_FC_Dot-plot
Example of surface staining pattern using Proaerolysin Alexa Fluor® 488
Sample: WT Jurkat cells spiked with GPI-deficient Jurkat cells
Staining protocol: 10 μl reagent / million cells in 100 μl of cell suspension; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD3 positive living Jurkat cells displayed as Proaerolysin Alexa Fluor® 488 versus CD90 APC
ED7751_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using Proaerolysin Alexa Fluor® 488
Sample: WT Jurkat cells spiked with GPI-deficient Jurkat cells
Staining protocol: 10 μl reagent / million cells in 100 μl of cell suspension; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: WT Jurkat cells
Negative events: GPI-deficient Jurkat cells
ED7751_FC_Profil Značení
Example of surface staining pattern using Proaerolysin Alexa Fluor® 488
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)

Product specific references:

Use of this product has not been published.
Variant
1 ml
ED7751
In stock
396.00 USD
Variant
1 ml
ED7751
In stock
396.00 USD