Exbio — Clinical products — Hematology — Single color antibodies — SingleFlowEx CD56 Pacific Blue™

SingleFlowEx CD56 Pacific Blue™

Regulatory status

RUO (GMP)

Antigen

CD56

Clone

LT56

Format

Pacific Blue™

Reactivity

Human

Application

FC (QC tested)

Excitation laser

violet (405 nm)

Variant

1 ml

ED7256

Delivery 1 week

440.00 USD

Variant

1 ml

ED7256

Delivery 1 week

440.00 USD

Contact distributor

Product details

Images

References

Isotype

Mouse IgG2a

Specificity

CD56

Application

FC (QC tested)

Reactivity

Human

Description

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Contents

1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.

Concentration

0.050 mg/ml

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

Example of surface staining pattern using SingleFlowEx CD56 Pacific Blue™
Clone: LT56
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx CD56 Pacific Blue™ versus CD3 APC

Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD56 Pacific Blue™
Clone: LT56
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: CD56 positive CD3 negative lymphocytes
Negative events: Neutrophil granulocytes