SingleFlowEx CD45RA PE-DyLight® 594

SingleFlowEx CD45RA PE-DyLight® 594
Regulatory status
RUO (GMP)
Antigen
CD45RA
Clone
MEM-56
Format
PE-DyLight® 594
Reactivity
Human
Application
FC
Excitation laser
blue (488 nm)
Variant
1 ml
ED7577
Delivery 1 week
500.00 USD
Variant
0.1 mg
11-261-C100
In stock
500.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2b
Specificity
CD45RA
Application
FC
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.040 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
ED7577_FC_Dot-plot
Example of surface staining pattern using SingleFlowEx CD45RA PE-DyLight® 594
Clone: MEM-56
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx CD45RA PE-DyLight® 594 versus CD3 APC
ED7577_FC_Dot-plot_2
Example of surface staining pattern using SingleFlowEx CD45RA PE-DyLight® 594
Clone: MEM-56
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx CD45RA PE-DyLight® 594 versus CD45R0 FITC
ED7577_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD45RA PE-DyLight® 594
Clone: MEM-56
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: CD45RA positive CD3 positive lymphocytes
Negative events: Neutrophil granulocytes
ED7577_FC_Profil Značení
Example of surface staining pattern using SingleFlowEx CD45RA PE-DyLight® 594
Clone: MEM-56
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)

Product specific references:

Use of this product has not been published.
Variant
1 ml
ED7577
Delivery 1 week
500.00 USD
Variant
0.1 mg
11-261-C100
In stock
500.00 USD