SingleFlowEx CD45R0 FITC

SingleFlowEx CD45R0 FITC
Regulatory status
RUO (GMP)
Antigen
CD45R0
Clone
UCHL1
Format
FITC
Reactivity
Human
Application
FC
Excitation laser
blue (488 nm)
Variant
1 ml
ED7569
Out of stock
360.00 USD
Variant
0.1 mg
11-261-C100
In stock
360.00 USD
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
CD45R0
Application
FC
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.080 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
The product is shipped with ice packs. Transport conditions, including short-term elevated temperature, do not impair product performance characteristics or do not affect stated product expiry date. Upon receipt, store and handle as directed.
Store at 2-8°C.
Keep away from sunlight.
Do not freeze.
Do not use after the expiry date stated on the product labels.
No reconstitution is necessary. Use the product directly from its original product container.
The product retains its performance characteristics until the expiry date when stored under stated conditions in its original product container.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
ED7569_FC_Dot-plot
Example of surface staining pattern using SingleFlowEx CD45R0 FITC
Clone: UCHL1
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx CD45R0 FITC versus CD3 APC
ED7569_FC_Dot-plot_2
Example of surface staining pattern using SingleFlowEx CD45R0 FITC
Clone: UCHL1
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx CD45R0 FITC versus CD45RA PE-Cy™7
ED7569_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD45R0 FITC
Clone: UCHL1
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: CD45R0 positive CD3 positive lymphocytes
Negative events: Neutrophil granulocytes
ED7569_FC_Profil Značení
Example of surface staining pattern using SingleFlowEx CD45R0 FITC
Clone: UCHL1
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)

Product specific references:

Kralickova P, Milota T, Litzman J, Malkusova I, Jilek D, Petanova J, Vidlakova J, Zimulova A, Fronkova E, Svaton M, Kanderova V, Bloomfeld M, Parackova Z, Klocperk A, Haviger J, Kalina T, Sediva A: CVID-associated tumors: Czech nationwide study focused on epidemiology, immunology, and genetic background in a cohort of patients with CVID. Frontiers in Immunology 2019, 9:3135
Variant
1 ml
ED7569
Out of stock
360.00 USD
Variant
0.1 mg
11-261-C100
In stock
360.00 USD