SingleFlowEx CD25 PE

SingleFlowEx CD25 PE
Regulatory status
RUO (GMP)
Antigen
CD25
Clone
MEM-181
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
1 ml
ED7116
Delivery 1 week
484.00 USD
Variant
1 ml
ED7116
Delivery 1 week
484.00 USD
Product details
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
CD25
Application
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.040 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
ED7116_FC_Dot-plot
Example of surface staining pattern using SingleFlowEx CD25 PE
Clone: MEM-181
Sample: PHA stimulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: PHA stimulated peripheral blood mononuclear cells displayed as SingleFlowEx CD25 PE versus CD3 APC
ED7116_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD25 PE
Clone: MEM-181
Sample: PHA stimulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: CD25 positive CD3 positive PHA stimulated lymphocytes
Negative events: CD25 negative CD3 negative PHA stimulated lymphocytes
ED7116_FC_Profil Značení
Example of surface staining pattern using SingleFlowEx CD25 PE
Clone: MEM-181
Sample: PHA stimulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)

Product specific references:

Cerny V, Hrdy J, Novotna O, Petraskova P, Borakova K, Kolarova L, Prokesova L: Distinct characteristics of Tregs of newborns of healthy and allergic mothers. Plos one 2018, 13(11):e0207998
Variant
1 ml
ED7116
Delivery 1 week
484.00 USD
Variant
1 ml
ED7116
Delivery 1 week
484.00 USD