SingleFlowEx CD15 FITC

SingleFlowEx CD15 FITC
Regulatory status
RUO (GMP)
Antigen
CD15
Clone
MEM-158
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
1 ml
ED7089
Delivery 1 week
396.00 USD
Variant
1 ml
ED7089
Delivery 1 week
396.00 USD
Product details
Images
References
SDS download
Isotype
Mouse IgM
Specificity
CD15
Application
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.050 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
ED7089_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD15 FITC
Clone: MEM-158
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: Neutrophil granulocytes
Negative events: CD15 negative lymphocytes
ED7089_FC_Profil Značení
Example of surface staining pattern using SingleFlowEx CD15 FITC
Clone: MEM-158
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)

Product specific references:

Marinov I, Illingworth AJ, Benko M, Sutherland DR: Performance characteristics of a non-fluorescent aerolysin-based paroxysmal nocturnal hemoglobinuria (PNH) assay for simultaneous evaluation of PNH neutrophils and PNH monocytes by flow cytometry, following published PNH guidelines. Cytometry B 2018, 94B:257-263
Variant
1 ml
ED7089
Delivery 1 week
396.00 USD
Variant
1 ml
ED7089
Delivery 1 week
396.00 USD