CD45 PerCP

CD45 PerCP
Regulatory status
CE IVD
Format
PerCP
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
ED7029
In stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
Product details
References
Application
FC (QC tested)
Reactivity
Human
Negative species
Horse
Description
The reagent CD45 PerCP permits identification and enumeration of cell populations expressing human CD45 antigen in whole blood using flow cytometry.
Contents
The reagent contains mouse monoclonal antibody against human CD45 antigen (clone MEM-28) which was purified by affinity chromatography and labeled with Peridinin-chlorophyll-protein complex (PerCP). The labeled antibody is diluted at optimum concentration in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. The content of a vial (2 ml) is sufficient for 100 tests.
Preparation
The purified antibody is conjugated with PerCP under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended for In Vitro Diagnostic Use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Product specific references:

Guttinger M et al. (1992) CD45 phosphotyrosine phosphatase and p56lck protein tyrosine kinase: a functional complex crucial in T cell signal transduction. Int Immunol. 4: 1325-30.
Stover DR et al. (1991) Protein-tyrosine-phosphatase CD45 is phosphorylated transiently on tyrosine upon activation of Jurkat T cells. Proc Natl Acad Sci USA 88: 7704-7707.
Taetle R et al. (1991) Regulation of CD45 expression in human leukemia cells. Leukemia 5: 309-314.
Nakano A et al. (1990) Expression of leukocyte common antigen (cd45) on various human leukemia/lymphoma cell lines. Acta Pathol Jpn. 40: 107-15.
Yamada A et al. (1990) Effect of activation of protein kinase C on CD45 isoform expression and CD45 protein tyrosine phosphatase activity in T cells. Eur J Immunol. 20: 1655-60.
Bazil V et al. (1989) Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression. Immunogenetics 29: 202-5
Horejsi V et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 34: 23-34.
Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).
Variant
100 tests
ED7029
In stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD