BasoFlowEx® Kit

BasoFlowEx® Kit
Regulatory status
CE IVD
Reactivity
Human
Application
FC (QC tested)
Variant
100 tests
ED7043
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
Images
Application
FC (QC tested)
Reactivity
Human
Description
The BasoFlowEx® Kit is intended for flow cytometry examination of IgE-mediated allergic reactions via analysis of CD63 antigen exposition on basophils in human heparinized whole blood upon allergen stimulation. Commercially available allergens can be used for the stimulation.
Contents
ED7043-1 Stimulation Buffer – 5 lyophilized vials, 1 vial is intended for stimulation of 20 tubes. ED7043-2 Stimulation Control – 2 lyophilized vials, 1 vial is intended for stimulation of 25 positive controls. ED7043-3 Staining Reagent – 1 vial containing 2 ml of premixed antibody cocktail: anti-CD63, FITC labeled + anti-CD203c, PE labeled. ED7043-4 Lysing Solution – 30 ml.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
ED7043 FC
Flow cytometric analysis of BasoFlowEx® Kit. (A) Visualization of compensated data on the side-scatter (SSC) versus forward scatter (FSC) in dot-plot. (B) Visualization of single cells gate on the FSC-Area versus FSC-height in dot-plot. (C) Visualization of basophil population (CD203 positive versus CD63 positive in dot-plot). (D) Visualization of stimulated (shown in red) and non-stimulated basophil population (shown in dashed line).
Variant
100 tests
ED7043
In stock
Variant
0.1 mg
11-261-C100
In stock

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