Exbio — Clinical products — Allergy — Kits — BasoFlowEx Kit

BasoFlowEx Kit

Regulatory status

CE IVD

Reactivity

Human

Application

FC (QC tested)

Variant

100 tests

ED7043

In stock

616.00 USD

Variant

100 tests

ED7043

In stock

616.00 USD

Contact distributor

Product details

Images

References

IFU download

SDS download

Application

FC (QC tested)

Reactivity

Human

Description

The BasoFlowEx Kit is intended for flow cytometry examination of IgE-mediated allergic reactions via analysis of CD63 antigen exposition on basophils in human heparinized whole blood upon allergen stimulation.
Commercially available allergens can be used for the stimulation.

Contents

ED7043-1 Stimulation Buffer – 5 lyophilized vials, 1 vial is intended for stimulation of 20 tubes.
ED7043-2 Stimulation Control – 2 lyophilized vials, 1 vial is intended for stimulation of 25 positive controls.
ED7043-3 Staining Reagent – 1 vial containing 2 ml of premixed antibody cocktail: anti-CD63, FITC labeled + anti-CD203c, PE labeled.
ED7043-4 Lysing Solution – 30 ml.

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended for In Vitro Diagnostic Use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Flow cytometric analysis of BasoFlowEx Kit. (A) Visualization of compensated data on the side-scatter (SSC) versus forward scatter (FSC) in dot-plot. (B) Visualization of single cells gate on the FSC-Area versus FSC-height in dot-plot. (C) Visualization of basophil population (CD203 positive versus CD63 positive in dot-plot). (D) Visualization of stimulated (shown in red) and non-stimulated basophil population (shown in dashed line).

Product specific references:

Rentzos G, Lundberg V, Lundqvist C, Rodrigues R, van Odijk J, Lundell AC, Pullerits T, Telemo E: Use of a basophil activation test as a complementary diagnostic tool in the diagnosis of severe peanut allergy in adults. Clin Transl Allergy. 2015 Jun 11;5:22. doi: 10.1186/s13601-015-0064-9.
PubMed

Depince-Berger AE, Sidi-Yahya K, Jeraiby M, Lambert C: Basophil activation test: Implementation and standardization between systems and between instruments. Cytometry A. 2017 Mar;91(3):261-269.
PubMed