Exbio — About exbio — Blog — Two newly added clones in our portfolio

Two newly added clones in our portfolio

DY12 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human CD245, and is suitable for flow cytometry, immunohistochemistry, immunoprecipitation, Western blotting and functional applications. CD245 is a 220-250 kDa transmembrane protein of unknown structure. It is expressed at lower level on resting blood lymphocytes, granulocytes, platelets, and thymocytes, and at high level on monocytes and on IL-2-dependent T cell lines. It is also expressed on Ewing sarcoma and osteosarcoma cell lines. CD245 seems to act as a costimulation molecule of T and NK cells.

Fig. 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD245 (DY12) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).

Currently available format:
Purified 11-941-C100
PE 1P-941-T100

15E3 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human CD303, and is suitable for flow cytometry, immunohistochemistry, immunocytochemistry, and ELISA. CD303 is an approximately 38 kDa type II transmembrane glycoprotein with an extracellular C-type lectin domain. It is a specific marker of plasmacytoid dendritic cells, and plays roles in capturing of pathogen-related oligosaccharide-containing antigens by them, and in their presentation to T cells. CD303 also mediates a potent inhibition of interferon alpha/beta production in plasmacytoid dendritic cells, thus it represents a potential target for lupus erythematosus therapy.

Fig. 2: Separation of human CD303 positive CD123 positive plasmacytoid dendritic cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD303 (15E3) purified antibody (concentration in sample 3 μg/ml, GAM FITC).

Currently available format:
Purified 11-943-C100
PE 1P-943-T100