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Two new interleukin-specific antibody clones

35C3 is a mouse monoclonal antibody, which recognizes human IL-2 (interleukin 2), and is suitable for flow cytometry, immunocytochemistry, immunoprecipitation, ELISA, and Western blotting. IL-2 is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein.

Fig. 1: Separation of human CD4 positive IL-2 positive lymphocytes (red-filled) from CD4 negative IL-2 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human PMA + ionomycin stimulated and Brefeldin A treated peripheral whole blood stained using anti-human IL-2 (35C3) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).

Currently available formats:
Purified 11-936-C100

9F9 is a mouse monoclonal antibody, which recognizes human IL-17A (interleukin 17A), and is suitable for flow cytometry, immunocytochemistry, immunoprecipitation, and ELISA. IL-17A is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. It plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It is the signature effector cytokine of Th17 cells, and in this role it primarily induces neutrophil activation and recruitment at infection and inflammatory sites. High levels of IL-17A are associated with rheumatoid arthritis, psoriasis, multiple sclerosis, and another inflammatory diseases, including lung injugy during severe COVID 19. This cytokine also contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. It is an effector cytokine for invariant NKT cells (iNKT), and it is involved in epithelial barrier formation upon injury.

Fig. 2: Separation of rare human CD4 positive IL-17A positive lymphocytes (red-filled) from CD4 negative IL-17A negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated and Brefeldin A treated peripheral whole blood stained using anti-human IL-17A (9F9) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).

Currently available formats:
Purified 11-937-C100