Two new antibody clones and two new polyclonals in our portfolio

6E3 is a mouse monoclonal antibody, which recognizes TIAR, an ubiquitously expressed approximately 42 kDa intracellular RNA-binding protein.

This antibody is suitable for flow cytometry, Western blotting, immunocytochemistry, and ELISA. TIAR regulates translational control, splicing, and other activities, including apoptosis, attenuates CDK1 activity, and is essential for the G2/M checkpoint. It accumulates in nuclear foci in late G2 phase and prophase in cells under replication stress. In steady state TIAR shuttles between the cytoplasm and the nucleus, probably as a part of nucleocytoplasmic transport of mRNA, but under stress conditions it accumulates mRNA molecules in granules and prevents their translation. Nucleolytic activity of TIAR against attacked target cells of cytotoxic lymphocytes has also been reported. Similarly, e.g. in permeabilized thymocytes TIAR triggers DNA fragmentation.

Currently available formats:

Anti-TIAR Purified
Anti-TIAR Alexa Fluor® 488
TIAR.jpg
FIG. 1:
Flow cytometry staining of TIAR in human cell line A-431 using purified mouse monoclonal antibody 6E3 (concentration in sample 5 μg/ml, GAM FITC, red-filled) from A-431 cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining).
 


B24 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD307b, and is suitable for flow cytometry.

CD307b is a type I transmembrane glycoprotein of the Fc receptor family. It contains one ITAM motif and two ITIM motifs in its cytoplasmic domain. It is expressed in spleen and lymph nodes in mature B cells and memory B cells. CD307b may be a prognostic marker for chronic lymphocytic leukemia.

Currently available formats:

Anti-Hu CD307b Purified
Coming soon: Anti-Hu CD307b PE
CD307b.jpg
FIG. 2:
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD307b (B24) purified antibody (0.5 μg/ml, GAM-FITC).



PAb (918) is a rabbit polyclonal antibody, which recognizes a red fluorescent protein tag mCherry, and is suitable for flow cytometry, immunocytochemistry, and Western blotting.

This fluorochrome has excitation maximum at 587 nm and emission maximum at 610 nm and is being used as a tag in expression systems.

Currently available formats:

Anti-mCherry purified
mCherry.jpg
FIG.3:
Separation of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mCherry/YFP cell suspension.



PAb (919) is a rabbit polyclonal antibody, which recognizes a far red fluorescent protein tag mPlum, and is suitable for flow cytometry, immunocytochemistry, and Western blotting.

This fluorochrome has excitation maximum at 589 nm and emission maximum at 650 nm and is being used as a tag in expression systems.

Currently available formats:

Anti-mPlum purified
mPlum.jpg
FIG.4:
Separation of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mPlum/YFP cell suspension.
 

Would you like to have our antibodies in other formates? Let us know!

Contact: info@exbio.cz