This antibody is suitable for flow cytometry, Western blotting, immunocytochemistry, and ELISA. TIAR regulates translational control, splicing, and other activities, including apoptosis, attenuates CDK1 activity, and is essential for the G2/M checkpoint. It accumulates in nuclear foci in late G2 phase and prophase in cells under replication stress. In steady state TIAR shuttles between the cytoplasm and the nucleus, probably as a part of nucleocytoplasmic transport of mRNA, but under stress conditions it accumulates mRNA molecules in granules and prevents their translation. Nucleolytic activity of TIAR against attacked target cells of cytotoxic lymphocytes has also been reported. Similarly, e.g. in permeabilized thymocytes TIAR triggers DNA fragmentation.
Anti-TIAR Alexa Fluor® 488
FIG. 1: Flow cytometry staining of TIAR in human cell line A-431 using purified mouse monoclonal antibody 6E3 (concentration in sample 5 μg/ml, GAM FITC, red-filled) from A-431 cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining).
CD307b is a type I transmembrane glycoprotein of the Fc receptor family. It contains one ITAM motif and two ITIM motifs in its cytoplasmic domain. It is expressed in spleen and lymph nodes in mature B cells and memory B cells. CD307b may be a prognostic marker for chronic lymphocytic leukemia.
Anti-Hu CD307b Purified
Coming soon: Anti-Hu CD307b PE
FIG. 2: Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD307b (B24) purified antibody (0.5 μg/ml, GAM-FITC).
This fluorochrome has excitation maximum at 587 nm and emission maximum at 610 nm and is being used as a tag in expression systems.
FIG.3: Separation of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mCherry/YFP cell suspension.
This fluorochrome has excitation maximum at 589 nm and emission maximum at 650 nm and is being used as a tag in expression systems.
FIG.4: Separation of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mPlum/YFP cell suspension.
Trop-2 is thought to be associated with the epithelial phenotype of cancer cells and many studies have reported that epithelial markers positively correlate with its expression, whereas mesenchymal markers typically exert negative correlation.
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. Among other applications, they also can be used as markers for detection and characterization of circulating tumor cells.