NK92.39 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD96, a transmembrane glycoprotein expressed at low levels on resting, and at high levels on activated T and NK cells, as well as on many T cell leukemias. This antibody is suitable for flow cytometry and functional application. CD96 is involved in adhesive interactions of T and NK cells during the late phase of the immune response. Strong interaction between CD96 and CD155 (poliovirus receptor) stimulates cytotoxicity of activated NK cells, which may play a role in NK cell recognition and killing of CD155-expressing tumor cells. CD96 is expressed on approximately 80% of T cell leukemias and 30% of acute myeloid leukemias and is being used for their immunophenotyping.
FIG. 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD96 (NK92.39) Purified antibody (concentration in sample 5 μg/ml, GAM APC).
SKII.4 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD155, a transmembrane glycoprotein, known as poliovirus and cytomegalovirus receptor. This antibody is suitable for flow cytometry, Western blotting, immunoprecipitation, and functional application. CD155 serves as an adhesion molecule involved in intercellular and cell-extracellular matrix interactions. It heterodimerizes with CD113 (nectin-3) and this complex binds to CD51/CD61 complex (integrin alphaVbeta3), and to vitronectin. CD155 is also thought to regulate CD44 binding to hyaluronan, and it is expressed on endothelial cells, epithelia, central nervous system, and monocytes.
FIG. 2: Separation of U937 stained using anti-human CD155 (SKII.4) purified antibody (concentration in sample 1.6 μg/ml, GAM-APC, red-filled) from U937 unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
15E2 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human CD369 (Dectin-1), a 33 kDa transmembrane glycoprotein expressed predominantly on dendritic cells. This antibody is suitable for flow cytometry and immunohistochemistry (frozen sections). CD369 serves as a part of innate immunity system by binding to beta-glucan polymers, which are typical for yeast and mycobacterial cell walls. CD369 is expressed predominantly on dendritic cells, but it can be detected also on monocytes, macrophages, mast cells, eosinophils, B cells, endothelial cells, and sometimes also on some T cell subsets.
FIG. 3: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD369 (15E2) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
When performing immunostaining procedures, it is important to know also the backround signal of the immunoglobulin itself, especially when higher antibody concentrations are being used. Hence negative controls represented by conjugates of corresponding antibody isotypes are available.
Today we introduce two mouse monoclonal antibody clones, that have been added to our portfolio: Anti-human CD79a (clone ZL7.4 - SURFACE STAINIG) and anti-human CD335 (clone 9E2).