5D12 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human NKp80 (CLEC5C), and is suitable for flow cytometry, immunoprecipitation, and functional applications. NKp80 is a type II transmembrane glycoprotein of the C lectin family, which is expressed in 80 kDa homodimers on NK cells, and subsets of CD8+ alpha/beta T cells, and gamma/delta T cells. It belongs to the activating coreceptors, which induce cytotoxicity, and production of pro-inflammatory cytokines. Its ligand AICL is expressed on myeloid cells.
Anti-Hu NKp80 Purified
Fig. 1: Separation of human NKp80 positive cells (red-filled) from human NKp80 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human NKp80 (5D12) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
K8 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD329, and is suitable for flow cytometry, Western blotting, immunocytochemistry, immunoprecipitation, and ELISA. CD329 is a type I transmembrane glycoprotein of siglec family, which contains an intracellular ITIM motif, and a SLAM-like motif, that acts as a docking site for SAP. CD329 can regulate TCR signaling by recruitment of SHP-1, which results in down-regulation of TCR-based gene transcription. It is expressed above all on monocytes, neutrophils, and a minor population of CD16+CD56- cells, weaker expression is detectable in some B cells, NK cells, and T cells.
Anti-Hu CD329 Purified
Fig. 2: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD329 (K8) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
42D1 is a rat monoclonal antibody, which recognizes an extracellular epitope of human CD85d, and is suitable for flow cytometry, and functional applications. CD85d is a type I transmembrane glycoprotein of the ILT/LIR family, which contains three intracellular ITIMs. It is expressed on NK cells, monocytes, macrophages, and weakly on granulocytes. It binds classic HLA class I molecules, and non-classic HLA-G1, and it is involved in the suppression of NK cell-mediated cytotoxicity. Interaction between CD85d and trophoblast-specific HLA-G1 probably contributes to maternal tolerance of the fetus by inhibiting maternal leukocytes. Antigen presenting cells, expressing CD85d and CD85k, appear to be crucial to the generation of CD8+ suppressor T cells and CD4+ regulatory T cells.
Anti-Hu CD85d Purified
Fig. 3: Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD85d (42D1) purified antibody (concentration in sample 1.7 μg/ml, DAR APC).
Dendritic cells represent a cornerstone of innate and adaptive immunity. We present here our antibodies to human dendritic cell markers, and a lineage cocktail for better gating of some dentritic cell types.
Exosomes have recently become a very popular topic. We present here our antibodies to exosomal markers.