M3/38 is a rat monoclonal antibody, which recognizes an N-terminal epitope of human and mouse galectin 3. This antibody is suitable for flow cytometry, Western blotting, immunoprecipitation, immunocytochemistry, and immunohistochemistry.
Galectin-3 is a galactose-binding lectin, which modulates intercellular interactions and interactions of the cell with ECM, as well as it is a nuclear protein and a component of inner mitochondrial membrane. Galectin-3 binds IgE, and takes part in formation of immunological synapse. It is detected cytoplasmatically in adenomas and carcinomas by immunohistochemistry. It is expressed in colonic and intestinal epithelium, papillary and follicular carcinomas, neoplastic astrocytes, inflammatory macrophages, and some lymphocytes. Upregulation of galectin-3 is involved in cancer progression and metastasis.
FIG.1: Separation of SK-MEL-30 cells stained using anti-galectin-3 (M3/38) purified antibody (concentration in sample 2.2 μg/ml, DAR APC, red-filled) from SK-MEL-30 cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of SK-MEL-30 cell suspension.
CW10 is a mouse monoclonal antibody, which recognizes an extracellular epitope on CD270, and is suitable for flow cytometry, Western blotting, immunoprecipitation, and immunocytochemistry .
CD270 is a type I transmembrane protein of the TNFR superfamily, which is expressed on resting T cells, monocytes, and immature dendritic cells. Its ligands, CD258 and CD272, differ in their effect on CD270 signaling. Whereas binding to CD258 provides a costimulatory signal, binding to CD272 gives to the cell an inhibitory signal. CD270 also is recognized by herpes simplex glycoprotein D. CD258-CD270 interaction and signaling is implicated in macrophage-derived foam cell-mediated development of atherosclerotic lesions.
Anti-Hu CD270 Purified
DT-9 is a mouse monoclonal antibody, which recognizes an extracellular epitope on HLA-C, whereas it does not crossreact with HLA-A or HLA-B allotypes. It is suitable for flow cytometry, and immunoprecipitation.
HLA-C, a member of MHC class I glycoproteins, is one of polymorphysm-typing targets, which are important for transplantation. The HLA system plays an important role in the occurrence and outcome of infectious diseases. The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes COVID-19, are reported to contain multiple class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population. It has been described that HLA-C interacts with human herpesvirus 8 MIR1 protein.
Fig.2: Separation of lymphocytes stained anti-human HLA-C (DT-9) purified antibody (concentration in the sample 1.7 μg/ml, GAM APC, red-filled) from lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Currently, Dr. Viktor Cerny and and Dr. Jiri Hrdy, together with their colleagues have published a paper Lower Functional and Proportional Characteristics of Cord Blood Treg of Male Newborns Compared with Female Newborns.
The terminal deoxynucleotidyl transferase (TdT) is a nuclear DNA polymerase that is primarily found in immature lymphoid cells of the B- and T-cell lineages