Three new antibody clones

CD85h.jpg24 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD85h, and is suitable for flow cytometry, immunohistochemistry, and functional applications. CD85h (leukocyte Ig-like receptor A2) is a type I transmembrane glycoprotein, associating with Fc receptor gamma chain, to serve as an activating immunoreceptor. In the result, however, CD85h signaling leads to suppression of dendritic cell differentiation and antigen presentation, and of innate immune responses. This glycoprotein is expressed mainly on monocytes, macrophages, and myeloid dendritic cells, at a lower level on granulocyte and NK cell subsets.
  
Fig. 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD85h (24) purified antibody (concentration in sample 0.56 μg/ml, GAM APC).
 
Currently available formats:
Purified               11-925-C100
 
 
11A8 is a mouse monoclonal antibody, which recognizes an extracellular epitope within human CD226, and is suitable for flow cytometry. CD226 is a type I transmembrane glycoprotein, which is expressed after activation on NK cells, platelets, monocytes, and some T cells. Its expression is increased in patients suffering from some autoimmune diseases or viral infections. CD226 is being phosphorylated on its cytoplasmic tail and interacts with LFA-1 complex (CD11a/CD18), resulting in calcium-independent intercellular contacts. Ligands for CD226 are CD155 and CD112. 
 
Currently available formats:
Purified               11-926-C100
 
 
CD325.jpg8C11 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human and mouse CD325 (N-cadherin), and is suitable for flow cytometry, Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, and functional applications. CD325 is a type I transmembrane protein, which forms a complex with catenins, that is linked to the actin cytoskeleton. This complex is important in synapses and for functional plasticity of neurons, and is also essential for embryonic development. Decreased CD325 cleavage caused by mutations in presenilin 1 is associated with Alzheimer´s disease. Besides nervous system, CD325 is expressed on the surface of malignant T cells, and increases their adhesion to epithelia, as well as their ability to invade and metastasize to inflammatory sites.
 

Fig. 2: Separation of HeLa cells stained using anti-CD325 (8C11) purified antibody (concentration in sample 3.3 μg/ml, GAM APC, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) antibody (concentration in sample 3.3 μg/ml, same as CD325 purified antibody concentration, GAM APC, black-dashed) in flow cytometry analysis (surface staining).
 
 
Currently available formats:
Purified               11-849-C100
 
 
 

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