Three new antibody clones

CD367.jpg9E8 is a mouse monoclonal antibody, which recognizes human CD367, and is suitable for flow cytometry, immunohistochemistry, and ELISA. CD367 is an approximately 20-28 kDa C-type lectin with immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic part. CD367 binds in calcium-dependent manner to mannose, fucose, and weakly also to N-acetylglucosamine. It is expressed on dendritic cells, macrophages, monocytes, B cells, and neutrophils. In rheumatoid arthitis patients CD367 is expressed also on CD4+ T cells. After ligand-mediated triggering, it is internalized by clathrin-dependent endocytosis and contributes to the antigen presentation to CD8+ T cells. It may also be involved in modulation of the antigen presenting cell response.
 

 
Fig. 1: Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD367 (9E8) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).
 
Currently available formats:
Purified                11-930-C100
 
 



PPM1D.jpg7E11/C5 is a mouse monoclonal antibody, which recognizes human and murine PPM1D, and is suitable for Western blotting, immunocytochemistry, immunohistochemistry, and ELISA. PPM1D is a Mg2+/Mn2+ dependent protein phosphatase 1D with inducible expression in response to various types of environmental stress. This expression is p53-dependent, and subsequently PPM1D negatively regulates the p53-mediated transcription, thus it suppresses the apoptosis. PPM1D contributes to development of carcinomas, and seems to be a promissing therapeutic target. Amplification of PPM1D is associated with breast cancer. 
 
 

 
Fig. 2: Western blotting analysis of human PPM1D using mouse monoclonal antibody 7E11/C5 on lysates of U2OS parental cells, expressing both natural (70 kDa, low expression) and C-terminally truncated version (55 kDa, high expression) of PPM1D, and on lysates of MCF7 cells (high PPM1D expression) and RPE cells (low PPM1D expression); reducing conditions.
 
Currently available formats:
Purified                11-894-C100
 
 



15C4 is a mouse monoclonal antibody, which recognizes human LOX1, and is suitable for flow cytometry, immunocytochemistry, and immunohistochemistry (frozen sections). LOX1 is a C-type lectin, functioning as a scavenger receptor for e.g. oxidized low density lipoprotein, apoptotic heat shock proteins, or CRP. It is expressed by macrophages, fibroblasts, platelets, endothelial cells, and smooth muscle cells, and its defects can lead to atherosclerosis. Its expression is enhanced under inflammatory conditions. Multiple splicing variants have been identified.
 
 
Currently available formats:
Purified                11-929-C100
 
Coming soon:
PE                          A4-929-C100
 
 

Would you like to have our antibodies in other formates? Let us know!

Contact: info@exbio.cz