Three new antibody clones

51.1 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD1d, and is suitable for flow cytometry, immunoprecipitation, Western blotting, immunohistochemistry, and functional applications.

 

cd1d.jpgCD1d belongs to CD1 family of transmembrane glycoproteins, associated with beta2 microglobulin, similarly to MHC I molecules. Unlike other CD1 family members, however, CD1d can be also expressed in a non-glycosylated form, which is not associated with beta2 microglobulin. Hence it is not certain how much CD1d plays a role in the presentation of microbial lipid antigens during infection. On the other hand, it is expressed on various antigen presenting cell types. Besides it, CD1d+ thymocytes are involved in the positive selection of sublineage of NKT cells.

Fig. 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD1d (51.1) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Currently available formats:

Anti-Hu CD1d Purified



3A6 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD166, and is suitable for flow cytometry, and immunohistochemistry.

 

CD166.jpgCD166 is a type 1 transmembrane glycoprotein, which is anchored to the actin cytoskeleton through its intracellular domain, whereas its extracellular part binds its ligand CD6. CD166 functions as an adhesion molecule expressed during development of many organs, as well as in hematopoiesis (pluripotent stem cells), endothelial and epithelial linings, endometrium, blastocysts, and in the central and peripheral nervous system (on neurons), as well as on activated T cells and activated monocytes. Multiple isoforms of CD166 have been found.

Fig. 2: Separation of HUVEC cells (red-filled) from human peripheral whole blood cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD166 (3A6) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).

Currently available formats:

Anti-Hu CD166 Purified

 

BM16 is a rat monoclonal antibody, which recognizes CD294, and is suitable for flow cytometry.

 

CD294.jpgCD294 (prostaglandin D2 receptor 2) is a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells, but also on eosinophils and basophils. It mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of CD294 gene have been associated with asthma susceptibility. Outside the immune system CD294 is expressed e.g. in gut, heart, and brain. The intracellular C terminal tail contains sites for phosphorylation by protein kinase C.

Fig. 3.: Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD294/CRTH2 (BM16) purified antibody (concentration in sample 5 μg/ml, GAR APC) and anti-human CD4 (MEM-241) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Currently available formats:

Anti-Hu CD294 Purified


 

Would you like to have our antibodies in other formates?

Let us know! Contact: info@exbio.cz