Standardized MRD Detection in Pediatric T-ALL

In flow cytometry, traditional liquid reagents can introduce variability due to handling, storage, and stability issues. EXBIO's Dry Reagents solve this by delivering custom multicolor antibody cocktails lyophilized directly into standard tubes—stable at room temperature, easy to use, and designed for consistent results.

A powerful example of their practical value comes from a recent multicenter study by the AIEOP-BFM-ALL-FLOW group, focused on standardizing flow-cytometric measurable residual disease (MRD) detection in pediatric T-acute lymphoblastic leukemia (T-ALL).

The researchers used custom-manufactured EXBIO dried antibody tubes containing a common backbone panel (e.g., CD45, CD99, CD48, CD8, CD5, CD7, CD3) in 8- and 12-color formats. These were tested across eight centers on 66 diagnostic and 67 day-15 samples from 81 patients, compared against local laboratory standards.
 

Key results:

• The dry tubes enabled correct blast identification in all diagnostic samples.
• On day-15 samples, MRD values showed excellent concordance with local methods (Spearman correlations of 0.94–0.98).
• Risk stratification agreement reached 90–93% at key cut-offs (0.1% and 10%).
• The dried format provided superior fluorochrome stability and uniform spillover, minimizing variability and supporting reliable multicenter comparison.

This approach—combining standardized protocols, guided analysis, and EXBIO's dry reagents—made reproducible MRD assessment possible in the vast majority of T-ALL cases, even for a rare and heterogeneous disease.

EXBIO Dry Reagents deliver the reliability and convenience that modern diagnostics and research demand. Interested in custom panels? Visit our Dry Reagents page or contact us today.

EXBIO team