PCNA – another useful marker of DNA synthesis

PCNA (proliferating cell nuclear antigen) is a DNA polymerase delta auxiliary protein, acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.
 

Schematic image of PCNA homotrimer interaction with DNA.

PCNA_schematic.jpg


 

Mouse monoclonal antibody PC10 detects PCNA of human, rat, mouse, chicken or Drosophila origin.

It can be used for immunocytochemistry, flow cytometry, Western blotting, immunohistochemistry and immunoprecipitation.



Currently available formats:

Anti-PCNA purified
Anti-PCNA PE

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Fig. 2:
Immunocytochemistry staining of PCNA in PHA-stimulated lymphocytes using mouse monoclonal antibody PC10 (diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green); DNA stained with propidium iodide (red). Colocalization of green PCNA signal and red DNA signal is indicated as yellow.

Fig. 3: Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-PCNA (PC10), 10 μg/ml. Commercially tested for EXBIO by LifeSpan BioSciences.

 

Would you like to have another formats of isotype controls sooner?

Let us know! Contact: info@exbio.cz