New Formats for Anti-HLA-G Sets

Why HLA-G Matters?

HLA-G, a non-classical MHC class I molecule, is mainly expressed at the maternal-fetal interface, promoting immune tolerance of the semi-allogeneic fetus. It modulates various immune responses. Tools like antibody G233 enable detailed study of its expression, relevant to pregnancy complications, fertility, and transplantation immunology. G233 is a highly specific, well-validated reagent critical for HLA-G research in placentation and maternal tolerance.

What G233 Recognizes?

G233 binds an extracellular epitope on multiple HLA-G isoforms (membrane-bound: G1–G4; soluble: G5–G7) without cross-reactivity to classical (HLA-A/B/C) or other non-classical (HLA-E/F) MHC class I molecules. This specificity is its major strength.
 

New formats of the G233 clone antibody.

Catalog #	Product name
1B-494-C100	Anti-HLA-G Biotin
1A-494-C100	Anti-HLA-G APC
1F-494-C100	Anti-HLA-G FITC
1P-494-C100	Anti-HLA-G PE

Combining with Other HLA-G Antibodies:

Combinations work best when pairing clones with complementary epitopes (native vs. denatured) and isoform coverage:

• MEM-G/9 — best partner for native HLA-G; binds surface HLA-G1, soluble HLA-G5, and HLA-G3 at a distinct epitope → enables co-staining with G233.
• 4H84 — detects denatured/free heavy chain (intracellular or non-β2m-associated forms); complements G233 surface staining, but cross-reacts with classical HLA-I free chains on activated cells → always confirm with G233.
• MEM-G/1 — covers denatured forms + native HLA-G2 (gap in G233/MEM-G/9); ideal for IHC on paraffin sections and HLA-G2-focused studies (e.g., tumors).

Recommended panel: G233 (native surface G1) + MEM-G/9 (native confirmation) + 4H84 (denatured/intracellular), add MEM-G/1 for G2 coverage. Use distinct fluorochromes; verify 4H84 with specific clones.

Key Applications of Fluorescent G233:

• Flow cytometry (primary use): surface & intracellular staining; quantifies HLA-G on trophoblasts (pregnancy) and tumor cells (hypoxia-induced, correlates with progression/metastasis; potential immune checkpoint).
• Immunofluorescence/ICC: visualizes subcellular localization (~10 µg/mL), membrane vs. intracellular isoforms.
• Advanced formats: terbium-based TR-FRET conjugate for high-throughput drug-discovery assays (target engagement, PPI).
• CyTOF: metal-tagged for high-parameter profiling in cancer/transplantation immunology.

Summary:

Fluorescent G233 is most powerful in flow cytometry for trophoblast, tumor, and immune-privileged site studies, supplemented by ICC/IF (localization), TR-FRET (screening), and CyTOF (complex phenotyping).

Would you like to have our antibodies in other formats? Let us know by e-mail or use our FORM!
Contact: info@exbio.cz