CD92 is a 70 kDa protein with ten transmembrane domains, intracellular N and C teminus, and two glycosylated larger extracellular loops. In the C-terminal domain, there is an ITIM-like sequence. This protein seems to be a choline transporter responsible for delivery of choline into the immune cells, to make it accessible for phospholipid synthesis, as well as a regulator of immune cell signaling. It is expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines. It can also be found on mast cells (but not eosinophils), and weakly on peripheral blood lymphocytes, fibroblasts, epithelial cells, and endothelial cells.
Anti-Hu CD92 Purified
Fig. 1: Separation of monocytes stained anti-human CD92 (VIM15) purified antibody (concentration in sample 0.6 μg/ml, GAM APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Anti-Hu CD15 Purified
Fig. 2: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD15 (W6D3) purified antibody (concentration in sample 15 μg/ml, GAM APC).
TCR Vgamma4 is a variant of TCR gamma chain, that is present on a minor subset of human gamma/delta T cells.
Anti-Hu TCR Vgamma4 Purified
Fig. 3: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Vgamma4 (4A11.904) purified antibody (concentration in sample 4 μg/ml, GAM APC).
TCR Vgamma9 is a variant of TCR gamma chain, that is present on a subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors.
Currently available formate:
Anti-Hu TCR Vgamma9 Purified
Fig. 4: Separation of human TCR Vgamma9 positive lymphocytes (red-filled) from human TCR Vgamma9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human TCR Vgamma9 (B3) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
European Commission extended deadlines for the implementation of the new IVDR (Regulation No. 2017/746) in order to avoid disruption of the supply of in vitro medical devices.
Accordingly to most recent studies, SCIMP appears to be a key, well defined component in initiation TLR-mediated pro-inflammatory responses in macrophages.
Today we introduce four mouse monoclonal antibody clones, that have been added to our portfolio: Anti-human TROP2 (clone TrMab-6), anti-human TCR gamma/delta (clone 11F2), anti-human CD272 (MIH26), anti-human granzyme A (CB9).