New clones in EXBIO portfolio

The mouse monoclonal antibody VIM15, recognizing an extracellular epitope of CD92, is suitable for flow cytometry and Western blotting.


CD92 is a 70 kDa protein with ten transmembrane domains, intracellular N and C teminus, and two glycosylated larger extracellular loops. In the C-terminal domain, there is an ITIM-like sequence. This protein seems to be a choline transporter responsible for delivery of choline into the immune cells, to make it accessible for phospholipid synthesis, as well as a regulator of immune cell signaling. It is expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines. It can also be found on mast cells (but not eosinophils), and weakly on peripheral blood lymphocytes, fibroblasts, epithelial cells, and endothelial cells.

Currently available formate:

Anti-Hu CD92 Purified
CD92.jpg
Fig. 1: Separation of monocytes stained anti-human CD92 (VIM15) purified antibody (concentration in sample 0.6 μg/ml, GAM APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).


Mouse monoclonal antibody W6D3 recognizes an extracellular epitope of CD15, and is suitable for flow cytometry. Importantly, this is a non-IgM anti-CD15 antibody.


CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.

Currently available formate:

Anti-Hu CD15 Purified
CD15.jpg
Fig. 2: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD15 (W6D3) purified antibody (concentration in sample 15 μg/ml, GAM APC).


The mouse monoclonal antibody 4A11.904 recognizes an extracellular epitope of human TCR Vgamma4, and is suitable for flow cytometry.


TCR Vgamma4 is a variant of TCR gamma chain, that is present on a minor subset of human gamma/delta T cells.

Currently available formate:

Anti-Hu TCR Vgamma4 Purified
TCRV-4.jpg
Fig. 3: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Vgamma4 (4A11.904) purified antibody (concentration in sample 4 μg/ml, GAM APC).


The mouse monoclonal antibody B3 recognizes an extracellular epitope of human TCR Vgamma9, and is suitable for flow cytometry.


TCR Vgamma9 is a variant of TCR gamma chain, that is present on a subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors.

Currently available formate:
Anti-Hu TCR Vgamma9 Purified
TCRV-9.jpg
Fig. 4: Separation of human TCR Vgamma9 positive lymphocytes (red-filled) from human TCR Vgamma9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human TCR Vgamma9 (B3) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
 

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Contact: info@exbio.cz