CD92 is a 70 kDa protein with ten transmembrane domains, intracellular N and C teminus, and two glycosylated larger extracellular loops. In the C-terminal domain, there is an ITIM-like sequence. This protein seems to be a choline transporter responsible for delivery of choline into the immune cells, to make it accessible for phospholipid synthesis, as well as a regulator of immune cell signaling. It is expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines. It can also be found on mast cells (but not eosinophils), and weakly on peripheral blood lymphocytes, fibroblasts, epithelial cells, and endothelial cells.
Anti-Hu CD92 Purified
Fig. 1: Separation of monocytes stained anti-human CD92 (VIM15) purified antibody (concentration in sample 0.6 μg/ml, GAM APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Anti-Hu CD15 Purified
Fig. 2: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD15 (W6D3) purified antibody (concentration in sample 15 μg/ml, GAM APC).
TCR Vgamma4 is a variant of TCR gamma chain, that is present on a minor subset of human gamma/delta T cells.
Anti-Hu TCR Vgamma4 Purified
Fig. 3: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Vgamma4 (4A11.904) purified antibody (concentration in sample 4 μg/ml, GAM APC).
TCR Vgamma9 is a variant of TCR gamma chain, that is present on a subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors.
Currently available formate:
Anti-Hu TCR Vgamma9 Purified
Fig. 4: Separation of human TCR Vgamma9 positive lymphocytes (red-filled) from human TCR Vgamma9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human TCR Vgamma9 (B3) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
We present here a brief comparison of TB3, UCHT1, and MEM-57 anti-CD3 flow cytometric antibodies, that have been submitted to HLDA workshops in past.
The monoclonal antibody JOVI.1 recognizes the TRBC1+ region and enables fast and reliable assessment of clonal restriction of TCR C genes by flow cytometry.
Today we introduce two mouse monoclonal antibody clones and two rabbit polyclonal antibodies, that have been added to our portfolio: Anti-TIAR (clone 6E3), anti-human CD300b (clone B24), polyclonal anti-mCherry, and polyclonal anti-mPlum.