OX-119 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD172g. This antibody is suitable for flow cytometry, and immunoprecipitation. CD172g is a transmembrane glycoprotein, which may play a role in inter-T cellular signaling by binding CD47, and thus in influencing T cell behaviour. CD172g is expressed on mature thymocytes, CD4+ T cells, CD8+ T cells, NK cells, and some B cells. It is absent on myeloid cells. Engagement of CD172g by CD47 expressed on antigen presenting cells results in enhanced antigen-specific T cell proliferation and costimulates T cell activation.
Anti-Hu CD172g Purified
Fig. 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD172g (OX-119) purified antibody (concentration in the sample 1.7 μg/ml, GAM APC).
YOL1/34 is a rat monoclonal antibody, which recognizes an epitope of alpha-tubulin in multiple species, especially in fixed state. This antibody is suitable for flow cytometry, Western blotting, immunohistochemistry, immunocytochemistry, and ELISA. The alpha-tubulin (relative molecular weight around 50 kDa) is a globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or is polymerized into microtubules. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. They are also necessary for movement of cells by means of flagella and cilia.
FIG. 2: Immunocytochemistry staining of alpha-tubulin in K562 cells using purified rat monoclonal antibody YOL1/34 (concentration in the sample 6 μg/ml, DAR FITC, left picture) vs. Hoechst 34580 nuclear staining (right picture).
IG191E/A8 is a mouse monoclonal antibody, which recognizes mouse and human BCL6. This antibody is suitable for flow cytometry, immunoprecipitation, Western blotting, and immunohistochemistry. BCL6 is a zinc finger-containing transcription factor, which acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. On B cells, BCL6 induces proliferation, antibody class switching and affinity maturation, whereas it inhibits their differentiation into plasma cells. On T cells, BCL6 induces their differentiation into T-FH cells.
FIG.3: Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human BCL6 (IG191E/A8) purified antibody (concentration in the sample 5 μg/ml, GAM APC).
We present here a brief comparison of TB3, UCHT1, and MEM-57 anti-CD3 flow cytometric antibodies, that have been submitted to HLDA workshops in past.
The monoclonal antibody JOVI.1 recognizes the TRBC1+ region and enables fast and reliable assessment of clonal restriction of TCR C genes by flow cytometry.
Today we introduce two mouse monoclonal antibody clones and two rabbit polyclonal antibodies, that have been added to our portfolio: Anti-TIAR (clone 6E3), anti-human CD300b (clone B24), polyclonal anti-mCherry, and polyclonal anti-mPlum.