New antibody clones in our portfolio

ZL7.4 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD79a (Ig alpha). This antibody is suitable for flow cytometry, immunocytochemistry, immunoprecipitation, ELISA, and immunohistochemistry (paraffin sections). CD79a forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Currently available formats:

Anti-Hu CD79a Purified
CD79a.jpg
FIG. 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD79a (ZL7.4) purified antibody (concentration in sample 5 μg/ml, GAM APC).



9E2 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human CD335. This antibody is suitable for flow cytometry. CD335 is a type I transmembrane glycoprotein, which serves as one of natural cytotoxicity receptors, and represents major receptor informing NK cells that they should kill particular virus-infected or tumor cell. Its sialic acids residues are involved in recognition of viral hemagglutinins, similarly it recognizes heparan sulphate proteoglycans on the surface of tumor cells. CD335 transfers the signal downstream via association with CD3 zeta chain, that contains intracellular ITAM motives. Expression of CD335, a specific marker of NK cells (both resting and activated), is down regulated by cortisol, and up-regulated by prolactin.

Currently available formats:

Anti-Hu CD335 Purified
CD335.jpg
FIG. 2: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD335 (9E2) purified antibody (concentration in sample 5 μg/ml, GAM APC).


Would you like to have our antibodies in other formates? Let us know!

Contact: info@exbio.cz