ZL7.4 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD79a (Ig alpha). This antibody is suitable for flow cytometry, immunocytochemistry, immunoprecipitation, ELISA, and immunohistochemistry (paraffin sections). CD79a forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
Anti-Hu CD79a Purified
FIG. 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD79a (ZL7.4) purified antibody (concentration in sample 5 μg/ml, GAM APC).
9E2 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human CD335. This antibody is suitable for flow cytometry. CD335 is a type I transmembrane glycoprotein, which serves as one of natural cytotoxicity receptors, and represents major receptor informing NK cells that they should kill particular virus-infected or tumor cell. Its sialic acids residues are involved in recognition of viral hemagglutinins, similarly it recognizes heparan sulphate proteoglycans on the surface of tumor cells. CD335 transfers the signal downstream via association with CD3 zeta chain, that contains intracellular ITAM motives. Expression of CD335, a specific marker of NK cells (both resting and activated), is down regulated by cortisol, and up-regulated by prolactin.
Anti-Hu CD335 Purified
FIG. 2: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD335 (9E2) purified antibody (concentration in sample 5 μg/ml, GAM APC).
When performing immunostaining procedures, it is important to know also the backround signal of the immunoglobulin itself, especially when higher antibody concentrations are being used. Hence negative controls represented by conjugates of corresponding antibody isotypes are available.
Easy way how to identify regulatory T cells (Tregs) in human peripheral blood or umbilical cord blood.