159 is a mouse monoclonal antibody, which recognizes SE domain of human histone deacetylase 6 (HDAC6), amino acids 971-981.
Crossreactivity with other species was not tested.
This antibody is suitable for Western blotting, immunocytochemistry, and ELISA.
Histone deacetylase 6 , like other histone deacetylases, affects gene expression by regulation of chromatin remodeling. It contains an internal duplication of two catalytic domains which appear to function independently of each other. Besides histones, HDAC6 deacetylates also other substrates including alpha tubulin and HSP90 alpha, and is involved in protein trafficking and degradation, as well as in affecting of cell shape and migration. Deregulation of HDAC6 expression and activity is associated with many diseases.
Fig. 1: Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 μg/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
23C6 is a mouse monoclonal antibody, which recognizes an extracellular epitope on CD51/CD61 complex.
This antibody reacts with human and chicken antigen, and is suitable for flow cytometry, immunoprecipitation, immunocytochemistry on frozen sections, and functional application (blocking).
The epitope is native and is sensitive to fixation.
CD51/CD61 complex is expressed mainly on human osteoclasts, but also e.g. on placenta, or melanoma cell lines. In chicken this antibody can be used to selectively identify the thrombocytes.
Fig. 2: Separation of MCF-7 cells stained using anti-CD51/CD61 (23c3) purified antibody (concentration in sample 1,7 μg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Anti-Hu CD51/CD61 Purified
When performing immunostaining procedures, it is important to know also the backround signal of the immunoglobulin itself, especially when higher antibody concentrations are being used. Hence negative controls represented by conjugates of corresponding antibody isotypes are available.
Today we introduce two mouse monoclonal antibody clones, that have been added to our portfolio: Anti-human CD79a (clone ZL7.4 - SURFACE STAINIG) and anti-human CD335 (clone 9E2).