Exbio — About exbio — Blog — New Anti-PAX5 Antibody

Anti-PAX5 Purified, clone 1H9

PAX5 (Paired box protein 5) is a transcription factor essential for B cell development and maintenance of B cell identity throughout differentiation. It's expressed from the pro-B cell stage through mature B cells but is lost upon plasma cell differentiation. PAX5 acts as both a transcriptional activator and repressor, controlling the expression of numerous B cell-specific genes while suppressing genes associated with other cell lineages.

The 1H9 clone is a well-characterized monoclonal antibody that specifically recognizes the PAX5 protein in both frozen and paraffinembedded tissues with high sensitivity and specificity. This antibody is extensively used in diagnostic histopathology for identifying B cell neoplasms, including B cell lymphomas and leukemias, and for distinguishing them from T cell malignancies and other tumors. It's also valuable in research studying B cell development, lymphomagenesis, and the molecular mechanisms controlling B cell differentiation and function.

	Fig. 1: Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-PAX5 (1H9) PE antibody (concentration in sample 0.56 μg/ml).
	Fig. 2: Separation of human PAX5 positive CD19 positive B cells (red-filled) from PAX5 negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-PAX5 (1H9) PE antibody (concentration in sample 0.56 μg/ml).
	Fig. 3: Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and intracellular staining using anti-PAX5 (1H9) PE antibody (concentration in sample 0.56 μg/ml).