New anti-Hu C3d Antibody (Clone BGRL11)

The complement fragment C3d is generated by cleavage of C3b and represents an important product of complement system activation. It covalently binds to the surface of pathogens or immune complexes, thereby ensuring their efficient recognition by the immune system. Its key role lies in the interaction with the CR2 (CD21) receptor on B lymphocytes, which strongly enhances the B cell response to antigen. The presence of C3d thus bridges innate and adaptive immunity, as CR2 signaling lowers the threshold required for B cell activation.  From a diagnostic perspective, C3d is used as a marker of complement activation, for example in tests for autoimmune hemolysis or in the study of immune complexes.
 
The BGRL11 clone produces a well-characterized monoclonal antibody that specifically recognizes human complement fragment C3d and shows minimal cross-reactivity with other complement components. It is meant primarily for use in flow cytometry, but is also widely used for AGG.  In indirect haemagglutination assays, BGRL11 agglutinates C3d on erythrocytes coated with C3 under low ionic strength and also agglutinates C3/C4-coated erythrocytes after alternative pathway activation. It is also capable of agglutinating C3d on erythrocytes from patients with cold haemagglutinin disease. BGRL 11 does not inhibit the classical activation pathway of complement mediated lysis by using blood group AB red cells sensitized by anti-A or B monoclonal antibodies.

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