2G3 is a mouse monoclonal antibody, which recognizes an extracellular/luminal epitope of CD207, and can be used for flow cytometry, immunohistochemistry, immunocytochemistry, and ELISA. CD207 (CLEC4K, langerin) is a 40 kDa type II transmembrane glycoprotein of the C-type lectin family. It binds to mannose-bearing glycoproteins and glycolipids of microbial and viral antigens, including HIV gp120. CD207 is expressed only in Langerhans cells, which are immature dendritic cells of the epidermis and mucosa, and its expression decreases during Langerhans cells maturation. Upon antigen binding CD207 localizes to the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed membranes, which leads to a nonclassical antigen-processing pathway. Fig. 1: Simplified schema of CD207 structure. Extracellular/luminal side up, intracellular side down. Fig. 2: Separation of human CD64 positive CD207 positive cells (red-filled) from CD64 negative CD207 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human stimulated (GM-CSF + IL-4 + TGF-beta) peripheral blood mononuclear cells stained using anti-human CD207 (2G3) purified antibody (concentration in sample 0.56 μg/ml, GAM APC). Currently available format: purified 11-946-C100 Coming soon: PE 1P-946-T100 Alexa Fluor 647 A6-946-T100
Contact: info@exbio.cz