CH21 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human CD158z (KIR3DL3), and can be used for flow cytometry and immunoprecipitation. CD158z is one of killer cell inhibitory receptors. It has three extracellular immunoglobulin-like domains and a long cytoplasmic tail, which, however, contains only one ITIM. Like other KIRs, CD158z is highly polymorphic, although it seems that its immunoglobulin-like domains are quite conserved among high primates. Interestingly, unlike most KIRs, CD158z is present in probably all human populations. Its ligand is B7H7, in the contrast to other KIRs, which recognize HLA class I molecules. To summ up, it seems CD158z plays a unique immunoregulatory role among KIRs. It gives a hope that it might be used as an immunotherapeutic target.
purified 11-945-C100 PE 1P-945-C100 APC 1A-945-C100 Fig. 1: Simplified schema of CD158z structure. Extracellular N-terminal part contains three immunoglobulin-like domains, and intracellular part contains a long C-terminal tail with one immunoreceptor tyrosine-based inhibitory motif. Fig. 2: Separation of CD158z positive cells (red-filled) from CD158z negative cells (black-dashed) in flow cytometry analysis (surface staining) of KIR3DL3 (CD158z) transfected HEK-293 suspension stained using anti-human CD158z (CH21) PE antibody (concentration in sample 5 μg/ml).
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