Tandem dye-to-monocyte non-specific binding is a widely documented undesirable phenomenon observed in multi-color flow cytometry. Tandem dye non-specific binding of monocytes complicates rare cell analysis and monocyte antigen analysis by increasing background of monocytes, virtually making monocytes falsely positive. This requires the use of additional monocyte markers and specific antibodies to be used in order to exclude falsely positive monocytes from analysis. We hereby present the importance and possibilities of prevention of such non-specific binding. EXBIO Praha a.s. has developed ED7747 Monocyte Blocking Buffer, a reagent intended to prevent non-specific monocyte-tandem dye interactions entirely. Monocyte blocking function has been confirmed by series of experiments. We also demonstrate that Monocyte Blocking Buffer does not affect binding of monoclonal antibodies reactive towards antigens expressed by monocytes. Figure 1: An overlay of staining profile of tested anti-CD19 conjugates in variant with Monocyte Blocking Buffer (red) and without Monocyte Blocking Buffer (black).
Our blog series about the very successful product DryFlowEx PNH High-Sensitivity Assay Kit continues.
CD340 (HER2, ErbB2) belongs to important cancer markers and targets for cancer therapy.