Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase, and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies. Mouse monoclonal antibody Ki-67 is that original clone, which has been used to identify and define Ki-67 antigen. It is suitable for Western blotting, immunohistochemistry, flow cytometry, and immunocytochemistry. It reacts also with bovine Ki-67 protein.
Fig. 1: Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue). Fig. 2: Flow cytometry analysis (surface staining) of HEK-293 cells with anti-Ki-67 (clone Ki-67) PE-Cy™7.
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