Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase, and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Mouse monoclonal antibody Ki-67 is that original clone, which has been used to identify and define Ki-67 antigen.
It is suitable for Western blotting, immunohistochemistry, flow cytometry, and immunocytochemistry. It reacts also with bovine Ki-67 protein.
Fig. 1: Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue).
Fig. 2: Flow cytometry analysis (surface staining) of HEK-293 cells with anti-Ki-67 (clone Ki-67) PE-Cy™7.
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Obviously, the present pandemic provides a strong impetus to deeper studies on mechanisms of pathologies caused by various respiratory viruses. Better understanding of this area may bring entirely novel approaches to prevention and therapy of diseases caused by such pathogens.
Here we present a short communication of Prof. Vaclav Horejsi, PhD (IMG AS CR) on this Hot Topic.
Fragmentation of DNA during apoptosis excludes apoptotic sperms from ability to give a life to a baby.