Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase, and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Mouse monoclonal antibody Ki-67 is that original clone, which has been used to identify and define Ki-67 antigen.
It is suitable for Western blotting, immunohistochemistry, flow cytometry, and immunocytochemistry. It reacts also with bovine Ki-67 protein.
Fig. 1: Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue).
Fig. 2: Flow cytometry analysis (surface staining) of HEK-293 cells with anti-Ki-67 (clone Ki-67) PE-Cy™7.
Currently, Dr. Viktor Cerny and and Dr. Jiri Hrdy, together with their colleagues have published a paper Lower Functional and Proportional Characteristics of Cord Blood Treg of Male Newborns Compared with Female Newborns.
Today we introduce one rat and two mouse monoclonal antibody clones, that have been added to our portfolio: Anti-galectin 3 (clone M3/38), anti-human CD270 (clone CW10), and anti-HLA-C (DT-9).