TrMab-6 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human TROP2. This antibody is suitable for flow cytometry, immunocytochemistry, immunohistochemistry and Western blotting. It is usefull for detection of TROP2 in breast cancer. TROP2 is a cell surface receptor that transduces calcium signals. It belongs to carcinoma-associated antigens. Mutations of TROP2 have been associated with gelatinous drop-like corneal dystrophy.
Fig.1: Separation of A431 cells (red-filled) from human peripheral whole blood cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human TROP2 (TrMab-6) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
Anti-Hu TROP2 Purified / Biotin / Alexa Fluor® 488 / APC
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11F2 is a mouse monoclonal antibody, which recognizes an extracellular epitope in all molecular forms of the TCR gamma/delta, and is suitable for flow cytometry, immunoprecipitation, immunohistochemistry (frozen sections), and ELISA. T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection.
Fig. 2: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
Anti-Hu TCR gamma/delta Purified
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MIH26 is a mouse monoclonal antibody, which recognizes an extracellular epitope on human CD272, and is suitable for flow cytometry and functional applications. CD272, a type I transmembrane glycoprotein, contains in its intracellular domain two ITIM sequences, which are upon CD272 triggering phosphorylated and recruit SHP phosphatases to attenuate cell activation. CD272 is expressed on B and T lymphocytes, NK cells, dendritic cells, and macrophages, and its ligand is CD270. Defects in CD272-CD270 inhibitory mechanism lead to autoimmune diseases. Overexpression of CD272 is a marker of tolerant T cells.
Fig. 3: Separation of human CD272 positive lymphocytes (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD272 (MIH26) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
Anti-Hu CD272 Purified
CB9 is a mouse monoclonal antibody, which recognizes human granzyme A, and is suitable for immunocytochemistry, flow cytometry, and immunoprecipitation. Granzyme A is a serine protease expressed in the cytoplasmic granules of T cells and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Similarly to granzyme B, granzyme A acts to destroy the target cells by proteolysis of their particular components. In case of granzyme A the targets are e.g. APEX1 (it destroys its oxidative repair activity), and nucleosome assembly protein SET (it disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA).
Fig. 4: Separation of human granzyme A positive NK cells (red-filled) from granzyme A negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using
anti-human granzyme A (CB9) purified antibody (concentration in sample 5 μg/ml, GAM APC).
Anti-Hu Granzyme A Purified
European Commission extended deadlines for the implementation of the new IVDR (Regulation No. 2017/746) in order to avoid disruption of the supply of in vitro medical devices.
Accordingly to most recent studies, SCIMP appears to be a key, well defined component in initiation TLR-mediated pro-inflammatory responses in macrophages.
Trop-2 is thought to be associated with the epithelial phenotype of cancer cells and many studies have reported that epithelial markers positively correlate with its expression, whereas mesenchymal markers typically exert negative correlation.