Four new antibody clones in our portfolio

Today we introduce four new antibody clones, that have been added to our portfolio.

 

B6.2 is a mouse monoclonal antibody, which recognizes a conformationally dependent epitope of native human CD66c.


Crossreactivity with other species was not tested. This antibody is suitable for flow cytometry and immunoprecipitation.

CD66c is a GPI-anchored glycoprotein capable of homophilic adhesion and heterophilic binding to CD66a-e, CD62E, and galectins. It is expressed on granulocytes and epithelial cells, and has potential applications in the detection of sites of infection and inflammation.

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Fig. 1: Separation of neutrophil granulocytes stained with anti-human CD66c (B6.2) purified antibody (concentration in the sample 3 μg/ml, GAM APC, red-filled) from neutrophil granulocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Currently available formats:

Anti-Hu CD66c Purified (Cat. No: 11-863-C100)
 

1D9-M12 is a mouse monoclonal antibody, which recognizes an extracellular epitope on human C5aR2 (C5a complement receptor).


This antibody is suitable for flow cytometry, Western blotting, and functional application (blocking).

C5aR2, also known as C5L2, is one of two receptors for C5a (anaphylatoxin). It is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages, and seems to have both pro-inflammatory and anti-inflammatory roles, depending on circumstances. Unlike CD88, C5aR2 is not coupled to G-protein, thus the modulatory role is more likely.

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Fig- 2: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C5aR2 (1D9-M12) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Currently available formats:

Anti-Hu C5aR2 Purified (Cat. No.: 11-880-C100)
 

V152 is a mouse monoclonal antibody, which recognizes mammalian cyclin B1.


This antibody is suitable for flow cytometry, Western blotting, immunocytochemistry, and immunohistochemistry (frozen sections, paraffin-embedded sections).

Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form maturation-promoting factor (MPF), and is necessary for proper control of the G2/M phase transition of the cell cycle. It is expressed in tissues containing proliferating cells, such as lymph node, testis, et al.


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Fig. 3: Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5 μg/ml, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Currently available formats:

Anti-Cyclin B1 Purified (Cat. No.: 11-871-C100)

 

hsF6.4.20 is a mouse monoclonal antibody, which recognizes an extracellular epitope on human CD352.


This antibody is suitable for flow cytometry, immunoprecipitation, and immunocytochemistry.

CD352, also known as SLAMF6 is a type I transmembrane glycoprotein expressed on NK cells, T cells, and B cells, and serves as a coreceptor for them. Besides association of its tyrosine phosphorylated intracellular domain with SH2D1A protein, it associates also with SH2 domain-containing phosphatases, which can modulate the signaling. Multiple CD352 isoforms have been identified.

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Fig. 4: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD352 (hsF6.4.20) Purified antibody (concentration in sample 0,56 μg/ml, GAM APC). 

Currently available formats:

Anti-Hu CD352 Purified (Cat. No.: 11-865-C100)
 

Would you like to have our antibodies in other formates? Let us know!

Contact: info@exbio.cz