Eight new antibody clones in our portfolio

CD49f.jpg
GoH3
is a rat monoclonal antibody, which recognizes an extracellular epitope of CD49f, and is suitable for flow cytometry, immunoprecipitation, immunocytochemistry, immunohistochemistry, and functional applications. CD49f (alpha 6 integrin) is a type I transmembrane glycoprotein, which noncovalently associates with CD29 (beta 1 integrin) to form VLA-6, and with CD104 (beta 4 integrin) to form alpha6/beta4 integrin complex. CD49f is expressed on platelets, megakaryocytes, monocytes, T cells and thymocytes. It is widely expressed on many cultured adherent cell lines and on epithelia in non-lymphoid tissues. It is important for adhesion to laminins, invasin, and merosin, hence also for cell migration, embryogenesis, metastasis, formation of hemidesmosomes in epithelia, and other processes.

Fig. 1: Separation of human blood thrombocytes stained using anti-CD49f (GoH3) purified antibody (concentration in sample 1.7 μg/ml, GAM APC, red-filled) from blood thromobocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Currently available formats:
Anti-CD49f Purified


CD74.jpg
LN2
is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD74 (the MHC II-associated invariant chain, Ii), and is suitable for flow cytometry, Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation. CD74 is a type II transmembrane protein expressed in antigen-presenting cells, that serves as MHC II chaperone which promotes MHC II trafficking from the ER to endocytic compartments, prevents peptide binding in the ER and contributes to peptide editing in the MHC II compartment. It is also an accessory signaling molecule implicated e.g. in malignant B cell proliferation. Stimulation of cell surface CD74 leads to NFkappaB activation, which enables entry of the stimulated cell into the S phase. CD74 binds pro-inflammatory cytokine MIF with high affinity and interacts with CD44. Binding of Vpu, an HIV1 protein, to CD74 modulates MHC II presentation.

Fig. 2: Separation of human CD74 positive lymphocytes (red-filled) from CD74 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD74 (LN2) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).

Currently available formats:
Anti-Hu CD74 Purified


CD75.jpg
LN1
is a mouse monoclonal antibody, which recognizes CD75, a lactosamine structure present mainly on the surface of B cell types, and is suitable for flow cytometry and immunohistochemistry. CD75 is downregulated during differentiation to plasma cells. It is being used as a marker which differentiates between malignant B cell types.

Fig. 3.: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD75 (LN1) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Currently available formats:
Anti-Hu CD75 Purified


CD85f.jpg
M4.1 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human CD85k, and is suitable for flow cytometry. CD85k is a type I transmembrane glycoprotein of ILT/LIR family, and contains three ITIM motives, which recruit protein tyrosine phosphatases SHP-1 and SHP-2, resulting in supression of signal transduction. It is expressed by monocytes, macrophages, and dendritic cells. Antigen presenting cells expressing CD85k and CD85d appear to be crucial to the generation of CD8+ suppressor T cells and CD4+ regulatory T cells.

Fig. 4: Flow cytometry multicolor surface staining of human peripheral blood mononuclear cells stained using anti-human CD85k (ZM4.1) purified antibody (concentration in sample 5 μg/ml, GAM APC) and anti-human CD123 (6H6) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Currently available formats:
Anti-Hu CD85k Purified



12D3 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD118, and is suitable for flow cytometry, Western blotting, ELISA and functional applications. CD118 (alpha subunit of LIF receptor) is a type I transmembrane glycoprotein, which associates noncovalently with CD130 to form the functional high affinity LIF receptor, which also acts as an oncostatin M receptor. CD118 alone binds LIF with low affinity. A secreted form of CD118 results from alternative splicing, and may have inhibitory effect, as it also binds LIF, although with low affinity. CD118 is not expressed on lymphocytes, but it is widely expressed outside the immune system. Soluble CD118 level rises during pregnancy, in parallel with a drop in circulating LIF levels.

Currently available formats:
Anti-Hu CD118 Purified


CD126.jpg
UV4
is a mouse monoclonal antibody, which recognizes an extacellular epitope of CD126, and is suitable for flow cytometry, immunoprecipitation, and Western blotting. CD126 is a type I plasma membrane glycoprotein of the hematopoietin receptor superfamily. It binds IL-6 and then associates with CD130 homodimers to form the functional IL-6 receptor. CD126 alone binds IL-6 with low affinity, and after association with CD130 with high affinity. It is expressed on normal B cells, T cells, monocytes, and hepatocytes, as well as on neoplastic (but not on normal) plasma cells. It is being used as an endothelial marker for separation of circulating endothelial cells from blood.

Fig. 5: Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD126 (UV4) purified antibody (concentration in sample 5 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Currently available formats:
Anti-Hu CD126 Purified


CD244.jpg
2-69 is a mouse monoclonal antibody, which recognizes an extracellular epitope of CD244, and is suitable for flow cytometry and immunoprecipitation. CD244 is a 70 kDa type I transmembrane glycoprotein containing four ITSM domains in its cytoplasmic part, serving as docking sites for both activating and inhibitory signaling molecules. Its extracellular ligand is CD48. CD244 is involved in non-MHC restricted cytotoxic activity of NK cells and acts as a costimulatory ligand for both NK cells and T cells. Interaction of CD244 with CD48 on T cells leads to the activation of both, but interaction with CD48 on other cells (e.g. tumor cells) can lead to inhibition of NK cell functions.

Fig. 6: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD244 (2-69) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Currently available formats:
Anti-Hu CD244 Purified


CD319.jpg
162 is a mouse monoclonal antibody, which recognizes an extracellular epitope of human CD319, and is suitable for flow cytometry, immunoprecipitation, immunohistochemistry, and functional applications. CD319 is a homophilic type I transmembrane protein with two intracellular immunoreceptor tyrosine-based switch motives (ITSMs). It is expressed by cytotoxic T cells, activated B cells, NK cells, and mature dendritic cells. CD319 triggers NK cell-mediated cytotoxicity. Ligation of CD319 can induce proliferation, cell adhesion, cytokine secretion, and cytotoxicity of T cells and NK cells.

Currently available formats:
Anti-Hu CD319 Purified
 
Fig. 7:
Separation of human CD319 positive lymphocytes (red-filled) from CD319 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD319 (162) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
 
 
 

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Contact: info@exbio.cz