HCDM (Human cell differentiation molecules) is an organisation which runs workshops on human leukocyte differentiation antigens, characterizes CD molecules and supplements their list.
Antibodies that have been submitted to HLDA workshops are well characterized and hence reliable for flow cytometry for research and mainly for diagnostic customers. We decided to compare three workshopped anti-CD3 monoclonal antibodies TB3, UCHT1, and MEM-57 regarding their concentration-dependent signal course. First, we conjugated them with Alexa Fluor® 647 to the same F/P ratio, and the conjugates were purified by size-exclusion chromatography. All three antibodies were then analysed in duplicates using flow cytometry on human peripheral blood, and 5-point titration curves were plotted. MFI, stain index, as well as percentage of CD3+ lymfocytes among all lymphocytes were evaluated.
Trop-2 is thought to be associated with the epithelial phenotype of cancer cells and many studies have reported that epithelial markers positively correlate with its expression, whereas mesenchymal markers typically exert negative correlation.
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. Among other applications, they also can be used as markers for detection and characterization of circulating tumor cells.