Exbio — About exbio — Blog — Comparison of anti-human CD38 clones HB7 and HIT2

Comparison of anti-human CD38 clones HB7 and HIT2

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration. CD38 belongs to markers of B cell subsets, leukemia, and a target of immunotherapy in myeloma treatment.

We used the anti-Hu CD38 clones HB7 and HIT2 FITC conjugates (1F-915-T100, 1F-366-T100, respectively) in 1.5 µg/ml concentration to evaluate their reactivity with particular cell populations of human peripheral leukocytes (fig. 1). The reactivity of both clones was comparable and their specificity was confirmed. We report here also comparison of these clones in particular on CD27⁺ B cells (fig. 2) and CD138⁺ B cells (fig. 3), where, also, both clones were fully comparable.

Fig. 1: Reactivity pattern of anti-CD38 clones HB7 and HIT2 in FITC format with corresponding populations of human peripheral blood leukocytes, namely neutrophils, eosinophils, basophils, classical monocytes, intermediate monocytes, non-classical monocytes, T cells, B cells, CD27^- B cells, memory B cells, plasma cells and NK cells.

Fig. 2: Reactivity pattern of anti-CD38 clones HB7 and HIT2 in FITC format on B cells, plotted against CD27.

Fig. 3: Reactivity pattern of anti-CD38 clones HB7 and HIT2 in FITC format on B cells, plotted against CD138.

Available formats of HB7 antibody:
purified (11-915-C100)
FITC (1F-915-T100)

Available formats of HIT2 antibody:
purified (11-366-C100)
biotin (1B-366-C100)
Pacific Blue™ (PB-366-T100, ED7145)
FITC (1F-366-T100, ED7146)
Pacific Orange™ (PO-366-T100)
PE (1P-366-T100, ED7147)
PE-DyLight® 594 (T5-366-T100)
APC (1A-366-T100)
PE-Cy™5 (T8-366-T100)
PerCP (PC-366-T100)
PerCP-Cy™5.5 (T9-366-T100, ED7193)
Alexa Fluor® 700 (A7-366-T100, ED7730)
PE-Cy™7 (T7-366-T100, ED7727)
APC-Cy™7 (T4-366-T100, ED7148)