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Comparison anti-human CD1a clones HI149 and SK9

CD1a, together with CD1b and c, serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent on vesicular acidification.

To get antigen presenting cells for comparison of anti-CD1a clones HI149 and SK9 binding, human peripheral blood monocytes were cultured for 5 days in the presence of GM-CSF and IL-4. We also used CD1a positive human acute T lymphoblastic leukemia MOLT-4 cell line and evaluated expression profile on adaptive immunity and innate immunity peripheral blood subsets (based on Kužílková D. et al., Front Immunol. 2022;13:827898). Working dilution of particular antibody conjugates was used according to the recommendations in the datasheets.

As expected, stimulated monocytes and MOLT-4 cells were detected by both anti-CD1a clones, but, interestingly, HI149 provided better signal on stimulated monocytes, whereas SK9 on MOLT-4 cells. Their reactivity pattern with particular adaptive immunity and innate immunity peripheral blood subsets was comparable.

Fig. 1: Comparison of anti-CD1a clones HI149 and SK9 concerning their reactivity with stimulated human monocytes. HI149 provided better signal.

Fig. 2: Comparison of anti-CD1a clones HI149 and SK9 concerning their reactivity with MOLT-4 cell line. SK9 provided better signal.

Fig. 3: Comparison of anti-CD1a clones HI149 and SK9 concerning their reactivity with adaptive immunity peripheral blood subsets.

Fig. 4: Comparison of anti-CD1a clones HI149 and SK9 concerning their reactivity with innate immunity peripheral blood subsets.

Links to anti-CD1a clones used in this study:
HI149
SK9