Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I and Illingworth A CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2020;98(2):179-192. doi:10.1002/cyto.b.21853
In this paper detection of CD71 (Transferrin I receptor) in immature RBCs (iRBCs) is utilized as an aid in PNH Type III, Type II and Type I clone identification in patients receiving blood transfusions. Healthy RBCs from a blood donor circulating in PNH patient`s bloodstream impact accurate PNH clone size percentage.
This paper was submitted by no other than Rob Sutherland himself. A friend and also a co-operator of ours when developing high quality reagents for leuko- and erythro- PNH analysis.
CD59 PE, CD71 APC and other cited antibodies used in this paper were kindly provided by EXBIO Praha after their successful development for this specific use in cooperation with Rob Sutherland.
Reference to EXBIO products:
CD15 antibody (MEM-158 clone)
CD59 antibody (MEM-43 clone)
CD64 antibody (10.1 clone)
CD71 antibody (MEM-75 clone)
CD157 antibody (SY11B5)
CD235a antibody (JC159 clone)
Most of you are familiar with Robs previous publications for high sensitivity PNH analysis on erythrocytes and lymphocytes.
In high sensitivity leukocyte analysis GPI-anchored antigens CD14 and CD157 are detected on monocytes and CD157 together with CD24 are detected on granulocytes. FLAER is used for further confirmation that the GPI anchor in PNH patients with PIG-A gene mutation is not present.
EXBIO Praha also kindly provided reagents used in high sensitivity leuko-PNH analysis published in:
Sutherland DR, Ortiz F, Quest G, et al. High-sensitivity 5-, 6-, and 7-color PNH WBC assays for both Canto II and Navios platforms. Cytometry B Clin Cytom. 2018;94(4):637-651. doi:10.1002/cyto.b.21626 Marinov I, Illingworth AJ, Benko M, Sutherland DR.
Marinov I, Illingworth AJ, Benko M and Sutherland DR Performance Characteristics of a Non-Fluorescent Aerolysin-Based Paroxysmal Nocturnal Hemoglobinuria (PNH) Assay for Simultaneous Evaluation of PNH Neutrophils and PNH Monocytes by Flow Cytometry, Following Published PNH Guidelines. Cytometry B Clin Cytom. 2018;94(2):257-263. doi:10.1002/cyto.b.21389
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