CD206 at a glance

CD206 is a transmembrane glycoprotein with C-type and B-type lectin functions. It is expressed at high levels by M2 macrophages, and besides them, it can be detected on immature dendritic cells, mesangial cells, hepatic and lymphatic endothelial cells, and in retinal pigment epithelium. Its main function is to recognize branched sugars with terminal mannose, fucose, or N-acetyl-glucosamine, typically expressed on the surface of microorganisms, and to mediate their phagocytosis. CD206 also serves for binding sulphated glycosyl residues of pituitary hormones, and particular sulphated glycoforms of certain CD molecules, such as CD169 and CD45. It seems to be also involved in scavenging and degradation of ricin.
 
In neural system, there CD206 is expressed on microglia and astrocytes, and plays a critical role at the beginning of recognition and capture of pathogens that have invaded the neural tissue. There is a positive correlation between the WHO pathological grades and the numbers of CD206-positive glioma-associated macrophages in human glioma tumor tissues. CD206 expression on induced microglia-like cells can serve as a biomarker not only in case of glioma, but also e.g. in monitoring bipolar disorder. Soluble CD206 (sCD206), released by metalloprotease-mediated cleavage,  can be used as a marker of macrophage activation, which helps to predict the severity of various diseases, including sepsis, pneumonia, cirrhosis, or autoimmunity diseases. Moreover, this soluble molecule still recognizes its mannosylated and sulphated ligands.

Fig. 1: Schematic image of CD206 glycoprotein.
 
CD206 has also become a target for immunotherapy. One of promissing approaches is to use analogs of host defense peptides that via selective conformational changes of CD206 and following signal transduction cause suppression of M2 phenotype of tumour-associated macrophages and lead to induction of their antitumor M1-like phenotype. 

The mouse monoclonal antibody 15-2 recognizes
an extracellular epitope of human CD206.

Applications: FC, IHC(F), ICC, WB, IP, FUNC (blocking)
HLDA workshop: VII, WS code 70802, MR15-2
Available formats:

Fig. 2: Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD11c (BU15) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).
 
 
Further reading:
 
Shen YW, Zhang YM, Huang ZG, Wang GC, Peng QL: Increased levels of soluble CD206 associated with rapidly progressive interstitial lung disease in patients with dermatomyositis. Mediators Inflamm. 2020 Oct 26;2020:7948095.
 
Tanaka S, Ohgidani M, Hata N, Inamine S, Sagata N, Shirouzu N, Mukae N, Suzuki SO, Hamasaki H, Hatae R, Sangatsuda Y, Fujioka Y, Takigawa K, Funakoshi Y, Iwaki T, Hosoi M, Lihara K, Mizoguchi M, Kato TA: CD206 expression in induced microglia-like cells from peripheral blood as a surrogate biomarker for the specific immune microenvironment of neurosurgical diseases including glioma. Front Immunol. 2021 Jun 29;12:670131.
 
Gage E, Hernandez MO, O'Hara JM, McCarthy EA, Mantis NJ: Role of the mannose receptor (CD206) in innate immunity to ricin toxin. Toxins (Basel). 2011 Sep;3(9):1131-45.
 
Stengel S, Quickert S, Lutz P, Ibidapo-Obe O, Steube A, Köse-Vogel N, Yarbakht M, Reuken PA, Busch M: Peritoneal level of CD206 associates with mortality and an inflammatory macrophage phenotype in patients with decompensated cirrhosis and spontaneous bacterial peritonitis. Gastroenterology 2020 May;158(6):1745-1761.
 
Wright PB, McDonald E, Bravo-Blas A, Baer HM, Heawood A, Bain CC, Mowat AM, Clay SL, Robertson EV, Morton F, Nijjar JS, Ijaz UZ, Milling SW, Gaya DR: The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease. Sci Rep. 2021 Oct 4;11(1):19616.
 
Tsuchiya K, Suzuki Y, Yoshimura K, Yasui H, Karayama M, Hozumi H, Furuhashi K, Enomoto N, Fujisawa T, Nakamura Y, Inui N, Yokomura K, Suda T: Macrophage mannose receptor CD206 predicts prognosis in community-acquired pneumonia. Sci Rep. 2019 Dec 10;9(1):18750.
 
Jaynes JM, Sable R, Ronzetti M, Bautista W, Knotts Z, Abisoye-Ogunniyan A, Li D, Calvo R, Dashnyam M, Singh A, Guerin T, White J, Ravichandran S, Kumar P, Talsania K, Chen V, Ghebremedhin A, Karanam B, Bin Salam A, Amin R, Odzorig T, Aiken T, Nguyen V, Bian Y, Zarif JC, de Groot AE, Mehta M, Fan L, Hu X, Simeonov A, Pate N, Abu-Asab M, Ferrer M, Southall N, Ock CY, Zhao Y, Lopez H, Kozlov S, de Val N, Yates CC, Baljinnyam B, Marugan J, Rudloff U: Mannose receptor (CD206) activation in tumor-associated macrophages enhances adaptive and innate antitumor immune responses. Sci Transl Med. 2020 Feb 12;12(530):eaax6337.
 

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