CD123 at a glance

CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.
 
High CD123 expression is detected in various hematological malignancies, especially in case of both acute and chronic myeloid leukemia, adult and childhood B-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, Hodgkin lymphoma, hairy cell leukemia, blastic plasmacytoid dendritic cells neoplasm, systemic mastocytosis and myelodysplastic syndrome. Lower expression can be detected in T-cell acute lymphoblastic leukemia, and in naturally CD123-expressing hematopoietic cells, plasmacytoid dendritic cells and endothelial cells.
                                                                                                                
CD123 is highly expressed on leukemic stem cells and serves as a marker to distinguish them from normal hematopoietic stem cells, and it is also expressed on more differentiated leukemic blasts. Hence it is being used for immunotherapy, mainly in case of AML, B-ALL, and BPDCN. Differences in antigen densities between leukemic and healthy cells are important for optimal therapeutic window. CD123 expression positively correlates with minimal residual disease after treatment and can be used as a marker of treatment success and a prognostic factor.

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Fig. 1: Schematic image of CD123 as a biomarker.
  

The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of human CD123.

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SingleFlowEx premium antibodies - application FC (QC tested)

SingleFlowEx CD123 FITC

SingleFlowEx CD123 PE
SingleFlowEx CD123 PerCP-Cy™5.5
SingleFlowEx CD123 PE-DyLight® 594
SingleFlowEx CD123 APC



anti-CD123.jpgResearch products - applications: FC, IHC(F), WB, IP
Available formats:
purified 11-700-C100
biotin 1B-700-C100
FITC 1F-700-T100
PE 1P-700-T100
APC 1A-700-T100
PE-Cy™5 T8-700-T100
PerCP-Cy™5.5 T9-700-T100
PE-Cy™7 T7-700-T100


Fig. 2: Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD123 (6H6) PE-Cy™7.
 
 
 
Further reading:
 
Liu K, Zhu M, Huang Y, Wei S, Xie J, Xiao Y: CD123 and its potential clinical application in leukemias. Life Sci. 2015 Feb 1;122:59-64.
 
El Achi H, Dupont E, Paul S, Khoury JD: CD123 as a biomarker in hematolymphoid malignancies: Principles of detection and targeted therapies. Cancers (Basel). 2020 Oct 23;12(11):3087.
 
Das N, Ritu R, Gupta SK, Bakhshi S, Malhotra A, Rai S, Singh S, Prajapati VK, Sahoo RK, Gogia A, Sharma A, Kumar L: A real-world perspective of CD123 expression in acute leukemia as promising biomarker to predict treatment outcome in B-ALL and AML. Clin Lymphoma Myeloma Leuk. 2020 Oct;20(10):e673-e684.
 
Jiang G, Atenafu EG, Capo-Chichi JM, Minden MD, Chang H: Prognostic relevance of CD123 expression in adult AML with normal karyotype. Br J Haematol. 2020 Jan;188(1):181-184.
 
Shi M, Su RJ, Parmar KP, Chaudhry R, Sun K, Rao J, Chen M: CD123: A novel biomarker for diagnosis and treatment of leukemia. Cardiovasc Hematol Disord Drug Targets. 2019;19(3):195-204.
 
Perriello VM, Gionfriddo I, Rossi R, Milano F, Mezzasoma F, Marra A, Spinelli O, Rambaldi A, Annibali O, Avvisati G, Di Raimondo F, Ascani S, Falini B, Martelli MP, Brunetti L: CD123 is consistently expressed on NPM1-mutated AML cells. Cancers (Basel). 2021 Jan 28;13(3):496.
 
Lamble AJ, Brodersen LE, Alonzo TA, Wang J, Pardo L, Sung L, Cooper TM, Kolb EA, Aplenc R, Tasian SK, Loken MR, Meshinchi S: CD123 expression is associated with high-risk disease characteristics in childhood acute myeloid leukemia: A report from the Children's oncology group. J Clin Oncol. 2022 Jan 20;40(3):252-261.
 
 

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