CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, cell survival, differentiation and other cell processes. It is also involved in melanogenesis and gametogenesis. After binding of its ligand, SCF (stem cell factor), CD117 is phosphorylated on its intracellular domain by neighbour CD117, which starts downstream signaling, leading to the effector functions. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells, anaplastic lymphomas, sarcomas, mastocytosis cells, melanoma, neuroblastoma, germinoma, gastrointestinal stromal tumours and other. These illnesses can be caused both by pathologically increased CD117 expression and by particular mutations that create pro-active conformation of mutated CD117, resulting in cancer cells proliferation. Hence, usage of tyrosine kinase inhibitors towards CD117 has strong clinical relevance. On the other hand, pathology is associated also with loss of CD117 function, as demonstrated e.g. by autosomal mottle disease. Fig. 1: Schematic drawing of CD117 activation in response to stem cell factor binding. Analogous conformational change can be induced also by particular mutations, resulting in oncogenic effect. Another mutations, however, can cause loss of CD117 functions. The mouse monoclonal antibody 104D2 recognizes an extracellular epitope of human CD117, and can be used for flow cytometry, immunocytochemistry, immunoprecipitation and immunohistochemistry. Currently available formats: Purified (11-586-C100), biotin (1B-586-C100), FITC (1F-586-T100, ED7174), PE (1P-586-T100, ED7175), APC (1A-586-T100), PE-Cy™5 (T8-586-T100), PerCP-Cy™5.5 (T9-586-T100, ED7176), Alexa Fluor® 700 (A7-586-T100, ED7601), PE-Cy™7 (T7-586-T100, ED7169), APC-Cy™7 (T4-586-T100, ED7177). Fig. 2: Identification of CD117+CD34+ stem cells in human peripheral blood using anti-CD117 (104D2) APC-Cy™7. Further reading: Sarlomo-Rikala M et al., Mod Pathol. 1998, 11(8):728-734. Kraj M et al., Clin Immunol. 2004, 29(1):1-9. Rassidakis GZ et al., Modern Pathol. 2004, 17:946-953. Miettinen M and Lasota J, Appl Immunohistochem Mol Morphol. 2005, 13(3):205-220. Schmidt-Hieber M et al., Haematologica 2011, 96(2):328-332. Liang J et al., Int J Biol Sci. 2013, 9:435-443. Tarlock K et al., Blood 2019, 134 (supplement 1):2699. Radu A et al., Exp Therapeutic Med. 2021, 21:78:1-9. Kaiser A et al., Blood 2021, 138 (supplement 1):2538.
Anti-human CD38 clones HB7 and HIT2 were compared regarding their reactivity with particular blood cell populations.
Here we present two basic systems of biotin detection, namely anti-biotin monoclonal antibody and streptavidin conjugates.
Anti-human CD25 clones MEM-181, B1.49.9, 2A3, BC96, CD25-4E3, and CD25-3G10 were compared regarding their reactivity with particular blood cell populations.