The α/ß T- cell receptor (TCR) is a transmembrane heterodimer expressed on >90 % normal T-cells and T-cell lymphomas.
The extracellular part of both α and ß chains comprises constant (C) and variable (V) regions.
The ß constant region contains 2 functionally identical but mutually exclusive isoforms: TRBC1 and TRBC2.
Normal T cells usually represent a mixture of TRBC1+ and TRBC2+ cells, while clonal T-cell population contains either TRBC1+ or TRBC2+ cells.
The monoclonal antibody JOVI.1 recognizes the TRBC1+ region and enables the assessment of clonal restriction of TCR C genes by flow cytometry with 97% sensitivity and 91% specificity.
Assessment of T- cell clonality by flow cytometry is comparable and could replace TCGR molecular study and shows reaching 100% sensitivity and 85% specificity for the diagnosis of LGLL.
Assoc. Prof. Iuri Marinov, CSc.
Head of the Laboratory of Flow Cytometry
Institute of Hematology and Blood Transfusion
We present here a brief comparison of TB3, UCHT1, and MEM-57 anti-CD3 flow cytometric antibodies, that have been submitted to HLDA workshops in past.
Today we introduce two mouse monoclonal antibody clones and two rabbit polyclonal antibodies, that have been added to our portfolio: Anti-TIAR (clone 6E3), anti-human CD300b (clone B24), polyclonal anti-mCherry, and polyclonal anti-mPlum.
Today we introduce two mouse monoclonal antibody clones, that have been added to our portfolio: Anti-human TCR Vdelta2 (clone B6), anti-human CD200R (clone OX-108).