Assessment of T- cell clonality by flow cytometry

The α/ß T- cell receptor (TCR) is a transmembrane heterodimer expressed on >90 % normal T-cells and T-cell lymphomas.

The extracellular part of both α and ß chains comprises constant (C) and variable (V) regions.

The ß constant region contains 2 functionally identical but mutually exclusive isoforms: TRBC1 and TRBC2.

Normal T cells usually represent a mixture of TRBC1+ and TRBC2+ cells, while clonal T-cell population contains either TRBC1+ or TRBC2+ cells.

The monoclonal antibody JOVI.1 recognizes the TRBC1+ region and enables the assessment of clonal restriction of TCR C genes by flow cytometry with 97% sensitivity and 91% specificity.

Assessment of T- cell clonality by flow cytometry is comparable and could replace TCGR molecular study and shows reaching 100% sensitivity and 85% specificity for the diagnosis of LGLL.





 

Assoc. Prof. Iuri Marinov, CSc.
Head of the Laboratory of Flow Cytometry
Institute of Hematology and Blood Transfusion

 

EXBIO PRODUCTS RELATED TO THIS BLOG:

The mouse monoclonal antibody JOVI.1 recognizing an extracellular epitope on TCR Cbeta1 (TRBC1) is available from EXBIO in the following formats:
 

Format:	Cat. No.:
Anti-Hu TCR Cbeta1 Purified	11-850-C100
Anti-Hu TCR Cbeta1 FITC	1F-850-C100
Anti-Hu TCR Cbeta1 PE	1P-850-C100
Anti-Hu TCR Cbeta1 APC	1A-850-C100

 

Would you like to have our antibodiy in other formates? Let us know!

Contact: info@exbio.cz